222 CHAPTER XVI. 



300. Methylen Blue and Eosin (CHENZINSKY, quoted from Zeit.f. 

 iviss. Mik., xi, 2, 1894, p. 260). 



Methylen blue, sol. sat. in water . . . 40 



Eosin, 0'5 per cent, in 70 per cent, alcohol . . 20 

 Distilled water, or glycerin . . . . .40 



This solution will only keep for about eight days. 



It has been recommended as a specific stain for blood. 



The mixture of PIANESK (Zeit.f. wiss. Mik., xi, 3, 1894, p. 345) contains 

 the same ingredients in the same proportions, with the addition of a con- 

 siderable proportion of carbonate of lithia. 



See also the mixture of BREMER, Arch.f. mik. Anat., xlv, 1895, p. 433 ; 

 or Zeit.f. wiss. Mik., xii, 3, 1896, p. 380. 



I have tried CHENZINSKY'S mixture as a tissue stain, without good re- 

 sults ; but see ROSIN, Berliner klin. Wochenschr., 1898, p. 251; Zeit.f. 

 wiss. Mik., xvi, 2, 1899, p. 223. 



301. Light Green (Litchgriin S. F.j. An acid colour, in 

 Ehrlich's sense, soluble in water or alcohol, and a good 

 plasma stain. 



BKNDA (Verlt. pliysiol. Ges. zu Berlin, Dec. 18th, 1891, 

 Nos. 4 u. 5 ; see also Zeit. f. wiss. Mik., viii, 1892, p. 516) 

 stains sections for twenty-four hours in anilin-water safranin 

 solution, then for about half a minute in a solution of 0*5 

 grin. Lichtgriiii or Saureviolett (Griibler) in 200 c.c. of 

 alcohol, dehydrates and mounts in balsam. This process 

 gives one of the most beautiful stains known to me. The 

 Saureviolett gives, perhaps, the more brilliant preparations, 

 and seems to be rather easier to carry out, as it may be 

 allowed to act rather longer than the Lichtgriiii. The pro- 

 cess in either form is a rather delicate one to cariy out, and 

 requires very thin sections. The Lichtgriiii stain unfor- 

 tunately does not keep very well ; I find my preparations 

 much faded after two years, but they will ker]> sufficiently 

 for many months at all events. 



302. Malachite Green (syn. Solid Green, Victoria Green, New 

 Green, Benzoyl Green, Fast Green). A basic colour, which has been 

 used as a plasma stain for the ova of Ascaris by VAN BENEDEN and NEYT 

 (see in Part II, " Cytological Methods "). These authors used it for glycerin 

 preparations. I have tried it for balsam sections, and find that it cannot be 

 used, as the stain is not sufficiently resistent to alcohol. 



303. Iodine Green (" HOFMANN'S Grim "), see GRIESBACH (Zool. Anz., 

 No. 117, vol. v, 1882, p. 40(5). The colour is now no longer manufactured 



ial purposes, but it is said may be obtained of excellent quality 



