MUSCLE AND TENDON (NERVE-ENDINGS). 375 



brings pieces for six to eight days (in the dark at first) into 

 a mixture of equal parts of -^ per cent, osmic acid and 

 ^ per cent, acetic acid, teases, and mounts in glycerin. 



For smooth muscle of Verines, see APATHY, Zeit. f. wiss. 

 Mik., x, 1893, pp. 36, 319. 



676. Smooth Muscle, Specific Stain for (UNNA, Monatssch.f. 

 prakt. Dermatol., xix, 1894, p. 533; Zeit. f. wiss. Mik., xii, 

 2, 1895, p. 243). Sections stained for ten minutes in 

 polychromatic methylen blue solution, rinsed in water, and 

 brought for ten minutes into 1 per cent, solution of red 

 prussiate of potash. This fixes the colour, so that the 

 sections will now bear differentiating with acid alcohol. 

 They are treated accordingly with alcohol acidified with 1 

 per cent, of hydrochloric acid for about ten minutes (until 

 the collagen ground comes out white). Absolute alcohol, 

 essence, balsam. 



In the same place see also another stain with acid orcein, 

 hgematein, Saurefuchsin, and picric acid. 



677. Iris (DOGIEL, Arch. f. mik. Anat., 1886, p. 403). An 

 enucleated eye is divided into halves, and the anterior one 

 with the iris brought for some days into a mixture of two 

 parts one third alcohol and one part 0'5 per cent, acetic 

 acid. The iris can then be isolated, and split from the 

 edge into an anterior and posterior plate, and these stained 

 according to the usual methods. 



See also KOGANEI, Arcli.f. mik. Anat., 1885, p. 1 ; Journ. 

 Roy. Mic. Soc., 1886, p. 874. 



Also CANFIELD, in Arch. f. mik. Anat., 1886, p. 121 ; and 

 DOSTOIEWSKY, ibid., p. 91. 



678. Bladder of Frog, Innervation of (WOLFF, Arch. f. mik. 

 Anat., 1881, p. 362). Impregnation with gold chloride 

 injected into the bladder through the anus. 



For details see previous editions. 



RANVIER (Traite, p. 854) also recommends one or the 

 other of his two gold processes, the bladder being carefully 

 distended by injection of the lemon-juice or gold chloride 

 and formic acid through the cloaca. 



