NEUROLOGICAL METHODS. 391 



clears with origanum oil. See also Jo urn. Roy. Mic. Soc., 

 1899, p. 443. 



LUITHLEN and SORGO (Neurol. Centralb., xvii, 1898, p. 640; 

 Zeit. f. iviss. Mik., xv, 1899, p. 359) differentiate in Unna's 

 glycerin-ether mixture (procurable from Griibler & Hollborn), 

 remove this with absolute alcohol and clear in origanum oil. 



EWING (Neiv York Med. Bee., 1898, p. 513; Zeit. f. u-iss. 

 Mik., xvi, 1899, p. 95) prefers to differentiate simply in 

 absolute alcohol, clear in oil of cajeput, and mount in balsam. 



See also some slight modifications in GOLDSCHEIDER & FLATAU, Nor male 

 und path. Anat. der Nervenzeilen, etc., Berlin, Komfeld, 1898; Zeit. f. 

 wiss. Mik., xvi, 1899, p. 102, and NISSL'S remarks thereon, Deutsche Zeit. 

 NervenheilJc., xiii, 1899, p. 348 ; Zeit.f. iviss. Milt., xvi, 1899, p. 370. 



Further, Cox, Intern. Monatsschr. Anat. Phys., xv, 1898, Heft 8 ; Zeit. 

 f. iviss. Mik., xvi, 1899, p. 101. 



696. Methylen Blue and Erythrosin. HELD (Arch. Anat. 

 Phys., Anat. Alth., 1895, 1896, p. 399) stains sections on 

 slides, with the aid of a gentle heat, for one or two minutes 

 in a solution of 1 grm. of Griibler's erythrosiii in 150 of 

 water with two drops of glacial acetic acid, washes out with 

 water, and stains in a mixture of equal parts of NissPs 

 methylen blue and 5 per cent, solution of acetone, warming 

 strongly the while, until all odour of acetone has disappeared. 

 After cooling he differentiates with O'l per cent, solution of 

 alum until the sections appear reddish, rinses in water, de- 

 hydrates as rapidly as possible in absolute alcohol, and passes 

 through xylol into balsam. For some slight modifications 

 see further HELD, op. cit., 1897, pp. 226233, 273305 

 (Supplementbaiid), and BOCCARDI, Mon. Zool. Ital., x, 1899, 

 p. 141 ; Zeit. f. wiss. Mik., xvi, 4, 1900, p. 471 (stains in a 

 mixture of erythrosiii O'l, toluidiii blue 0'2, and water 100 

 parts, and differentiates in 0'5 per cent, alum solution). 



697. Thionin. LENHOSSEE (Fein. Ban. d. Nerven systems, 

 Berlin, 1894, p. 149) stains sections of formol material for 

 five minutes in a concentrated aqueous solution of thioiiin, 

 rinses with water, differentiates in a mixture of 1 part aniliii 

 oil to 9 of absolute alcohol, and passes through oil of cajeput 

 or xylol into damar or balsam. The stain does not keep well. 



Similarly RAMON Y CAJAL. Man. de Anat. Path. Gen., 1896 



