CHAPTER XXXII. 



NEUROLOGICAL METHODS, AX1S-CYLTNDEE AND PROTOPLASM 

 STAINS (GOLGI AND OTHERS). 



730. Introduction. There are three chief methods for the 

 study of axis-cylinders and protoplasmic nerve-cell processes, 

 viz. the methyleii-blue staining method, the sublimate method 

 of GOLGI, and the bichromate-of-silver method of GOLGI. The 

 methylen-blue method having been given in Chap. XVII, it 

 remains to group together here some other subordinate but 

 useful methods that are also stains proper ; after which will 

 be given the methods of GOLGI and some other impregnation 

 methods. 



(c) Stains Proper. 



731. Anilin blue-black has been mucli recommended by SANKEY 

 (Quart. Journ. Mic. Sci., 1876, p. 69) ; BEVAN LEWIS (Human Brain, 

 p. 125 ; VEJAS (Arch. f. Psychiatric, xvi, p. 200) ; GIERKE (Zeit. f. iviss. 

 Mik., 1884, p. 376) ; MARTINOTTI (ibid., p. 478) ; JELGERSMA (Zeit.f. wiss. 

 Mik., 1886, p. 39) ; SCHMAUS (Munch, med. Wochenschr., No. 8, 1891, 

 p. 147; Zeit.f. wiss. Mik., viii, 1891, p. 230), and others. I have not been 

 able to identify the colour used by these authors, but as they concur in 

 saying that the English preparation sold under that name alone gives good 

 results, I conclude that it must have been the anilin black of Lightfoot. 

 If so, it is no longer found in commerce, and should not be quoted as a his- 

 tological reagent (see 313). For details see previous editions. 



732. MAHTINOTTI (loc. cit., 1884, p. 478) linds that picro- 

 iiigrosin gives very good results, especially for pathological 

 objects. He stains for two or three hours or days in a 

 saturated solution of iiigrosin in saturated solution of picric 

 acid in alcohol, and washes out in a mixture of 1 part of 

 formic acid with 2 parts of alcohol until the o-rey matter 



O / 



