NEUROLOGICAL METHODS. 411 



minutes in water, and stained for twenty-four hours in a 

 solution of 2 grammes of hagmatoxylin (dissolved in a little 

 alcohol) in 100 c.c. of 2 per cent, acetic acid. They are 

 washed out until they are of a light blue-red colour in 80 per 

 cent, alcohol acidulated with 0'5 per cent, of hydrochloric 

 acid. Remove the acid thoroughly by washing with pure 

 alcohol, dehydrate,, clear with origanum oil, and mount. 



A sharp axis-cylinder stain, myelin being coloured only if 

 the differentiation in the acid alcohol is insufficient. 



737. SCARPATETTI (Neurol. Centralb., xvi, 1897, p. 211 ; Zeit. f. wiss. 

 Mik., xiv, 1897, p. 91) obtains an axis-cylinder stain as follows : Sections 

 of material hardened in 5 to 10 per cent, formol, followed by alcohol, are 

 stained for five minutes in 1 per cent, hsematoxylin, treated for five minutes 

 with concentrated solution of neutral acetate of copper, differentiated witli 

 Weigert's borax-ferricyanide, then treated with concentrated solution of 

 carbonate of lithia, washed, and mounted. Myelin is not stained. 



738. Iron-Haeniatoxyiin sometimes gives an axis-cylinder 

 stain. 



739. MALLORY'S Phospho-molybdic-acid Haematoxylin has been 

 given, 259, where see also that of KKXYON. 



For the extremely complicated modification of AUEBLACH, see Neurol. 

 Centralb., xvi, 1897, p. 439, or Zeit. f. wiss. Mik., xiv, 1897, p. 402. 



740. FIXOTTI (Virchow's Arch., cxliii, 1896, p. 133; Zeit. f. wiss. Mik., 

 xiii, 1896, p. 236) stains in hrematoxylin, washes out well, counter-stains 

 for three minutes with O'o to 1 per cent, solution of Sauret'uchsin, and 

 differentiates in 75 per cent, alcohol containing a very little caustic potash. 

 See also 704. 



741. ALT (Miincli. med. Wochenschr., 1892, No. 4; Zeit.f. wiss. Mik., 

 ix, 1, 1892, p. 81) stains for a couple of hours in solution of Congo in abso- 

 lute alcohol, and, washes out with pure alcohol. The results are said to be 

 specially adapted to the study of peripheral axis-cvlinders. 



742. APATHY'S methods. See $ 707. 



(d) Impregnations. 



743. The Methods of GOLGI. There are two methods of 

 GTOLGI, viz. the Corrosive Sublimate Method and the Bichromate 



