432 CHAPTKR XXXII. 



properly performed : amongst them liquid of Flemming, and 

 that of Miiller. Formaldehyde mixtures he does not recom- 

 mend. 



LEBER (Milncli. med. Wochenschr., xli, 30, 1894; cf. Zeit.f. wiss. Mik., 

 xii, 1895, p. 256) confirms Hermann's observation concerning eyes (see 

 p. 76). He advises a 4 per cent, solution (formol 1, water 10). After a 

 few days' hardening in this, the eyes may Le cut through, it is said, 

 without derangement of the parts. The retina lies flat, and is at least as 

 well preserved as with solution of Miiller. The eyes may be passed with- 

 out hurt direct into successive alcohols ; the vitreous will shrink a little, 

 but less rather than more than after solution of Miiller. I doubt the 

 correct cytological preservation of the elements by this process. 



See also HIPPEL (Arch. f. Oplitlialm., xlv, 1898, p. 286; Zeit. f. wiss. 

 Mile., xvi, 1, 1899, p. 79), who finds that formol fixes the lens badly, the 

 retina well, so far at least as the absence of folds from shrinkage is con- 

 cerned. 



780. Staining. RAMON Y CAJAL employs the rapid method 

 of GrOi.Gi, double-impregnation process, 749. 



KUHNT (Jen. Zeit.f. Natunc., Bd. xxiv, H. 1, 1889, p. 177 ; 

 Zeit. f. wiss. Mik., vii, 1, 1890, p. 65) employs Pal's modifi- 

 cation of Weigert's hsematoxylin process. 



DOGIEL employs the methylen-blue method, M26, 328, 

 330. 



SCHAFKKR (Sitzb. k. Akad. Wiss. Wien, xcix, 1890, 3, p. 110; 

 Zeit. f. iviss. Mik.. viii, 2, 1891, 227) recommends mordant- 

 ing sections in 1 per cent, chromic acid for some hours, 

 washing for a short time only with water, staining for twenty 

 hours in Kultschitzky's acetic acid ha3inatoxylin ( 714), and 

 differentiating for twelve hours in Weigert's ferricyanide 

 .solution ( 710). 



KRAUSE (loc. cit., 782) treats fresh retina with perchloride of iron or of 

 vanadium in 1 per cent, solution, and then with a 2 per cent, solution of 

 tannic or pyrogallic acid. These reagents only stain the granule layers 

 and the nuclei of the ganglion cells. The elements of the other layers may 

 then be stained with Saurefuchsin or some other anilin. 



LENNOX (Arch. f. Ophthalm., xxxii, 1 ; Zeit. f. wiss. Mik., iii, 3, 1886, 

 p. 408 ; and Journ. Hoy. Mic. Soc., 1887, p. 339) has applied Weigert's 

 luematoxylin method to the retina. 



CUCCATI stains with concentrated aqueous solution of Saurefuchsin, and 

 mounts in balsam . 



See also BERNHEIMER, Sb. k. Akad. Wiss. Wien, 1884; or Journ. Roy. 

 Mic. Soc., 1886, p. 167 ; and RAMON Y CAJAL, Rev. trim, de Hist. Norm. // 



