436 CHAPTKR XXXIII. 



RAMON Y CAJAL'S picro-indigo-carmine stain has been given, 

 374. 



RIBBEET (Zeit. f. wiss. Mik., xv, 1898, p. 93) recommends MALLOEY'S 

 phospho-rnolybdic acid hsernatoxylin ( 259) with previous mordanting for 

 half a minute in the phospho-inolybdic acid, then rinsing, and staining foi 

 five minutes. 



BENECKE'S Stain for Fibrils (Verh. d. auat. Ges., vii Vers., 

 1893, p. 165 ; Zeit.f. wiss. Mik., xi, 1, 1894, p. 79) is essen- 

 tially that of KKOMAYER ( 656). 



See also KEOMAYEE, Dermat. Zeit., iii, 1896, p. 263 ; Zeit.f. iviss. Mik., 

 xiv, 1897, p. 56. 



787. UNNA (Monatssch. f. prakt. Dermatol., xviii, 1894, p. 

 509 ; Zeit. f. iviss. Mik., xi, 4, 1894, p. 518) finds that the 

 method of BKNECKE (last ) is unsurpassed for the demonstra- 

 tion of collagen, fibrils alone, but prefers the following 

 whenever it is desirable to obtain at the same time good 

 images of the ground-substance and of other elements of 

 preparations. 



1. The Orcein Method. Sections of alcohol material are 

 stained for five minutes in Grubler's strong solution of poly- 

 chromatic methylen blue. They are then brought for fifteen 

 minutes into a neutral 1 per cent, solution of orcein in abso- 

 lute alcohol, rinsed in pure alcohol, cleared in bergamot oil, 

 ,-ind mounted. Nuclei blue, collagenous ground-substance 

 dark red, granules of Mastzellen carmine red, protoplasm of 

 Plasmazellen blue. 



2. The Method of Sulphosalts. (a) Stain for five or ten 

 minutes in an aqueous 2 per cent, solution of Saurefuchsin, 

 rinse, treat for one or two minutes with saturated aqueous 

 solution of picric acid, dehydrate (two minutes) in absolute 

 alcohol saturated with picric acid, rinse with pure alcohol, 

 clear and mount. (&) Stain for twenty seconds in aqueous 

 1 per cent, solution of Wasserblau, rinse, treat for five 

 minutes with neutral aqueous 1 per cent, solution of safranin. 

 Rinse and put into absolute alcohol until the blue colour re- 

 appears, clear with bergamot oil and mount. Collagen light 

 blue, nuclei red. 



