SOME OTHKR HISTOLOGTCAL METHODS. 441 



For the elastic tissue of the skin, see PASSARGE and KROSING, Dermat. 

 .Stud., xviii, 1894. 



See also for staining and dissociation AGABABOW, Arch. mik. Anat., 1, 

 1897, p. 566, et seq. 



For C. MARTINOTTI'S silver impregnation, see Zeit.f. iviss. Mik., v, 1888, 

 p. 521, or Arch. Ital. Biol, xi, 1889, p. 257. 



SCHUMACHER (Arch. mile. Anat., lv, 1899, p. 151, Zeit.f. iviss.Mik., xvi, 

 4, 1900, p. 456) has had good results (for the spleen) with a mixture of ] 

 part 1 per cent, nigrosin and 9 parts saturated aqueous picric acid. 



See also 821. 



799. UNNA'S Modified Orcein Method (Monatssch. f. prakt. 

 DermatoL, xix, 1894, p. 397; Zeit.f. u<i*s. Mik., xii, 2, 1895, 

 p. 240). The following solution is made : Griibler's orceiii 

 1 part, hydrochloric acid 1 part, absolute alcohol 100 parts. 

 The sections are put into a porcelain capsule with just 

 enough of the stain to cover them, and the whole is warmed 

 in a stove or over a naked Hame to about 30 C. After ten 

 to fifteen minutes the stain becomes quite thick, owing to 

 evaporation of the alcohol. The sections are then well 

 rinsed in alcohol, cleared, and mounted. Elastin dark brown, 

 collagen light brown. 



For UNNA'S earlier orcein method, see third edition, or Monatssch. f. 

 prakt. DermatoL, xii, 1891, p. 394 (Zeit.f. wiss. Mik., ix, 1, 1892, p. 94). 



See also ZENTHOEFER, in Unna's DermatoL Studien, 1892, or Zeit. f. 

 wiss. Mik., ix, 4, 1893, p. 509 ; KOPPEN, Zeit. f. iviss. Mik., vi, 4, 1889, 

 p. 473 ; and vi, 1, 1890, p. 22, or third edition ; BTJRCI, Journ. Boy. Mic. 

 Soc., 1891, p. 831, and 1892, p. 292, or third edition ; HANSEN, Virchow's 

 .Archiv, cxxxvii, 1894, p. 25; Zeit.f. tviss. Mik., xi, 3, 1894, p. 383; 

 KULTSCHIZKY, ibid., xiii, 1, 1896, p. 74, or the original, Arch.f. mik. Anat., 

 xlvi, 1895, p. 673 ; GUNTHER, Zeit. f. wiss. Mik., xiii, 1896, p. 230 ; 



SCHIEFFERDECKER, ibid., p. 302 ; TfilEPEL, ibid., XIV, 1897, p. 31 ; LOISEL, 



Journ. de VAnat., et de la Phys., xxxiii, 1897, p. 134; GARDNER, Biol. 

 Central^., xvii, 1897, p. 398 ; LIVINI, Monitore Zool. Ital., vii, 1896, p. 45 

 (Journ. Boy. Mic. Soc., 1899, p. 455). 



800. WEIGEET'S Fuchsin-resorcin Method (Zeit. f. wins. Mik., 

 xvi, 1899, p. 81). 1 per cent, of basic fuchsin and 2 per 

 cent, of resorcin (or of carbolic acid) are dissolved in 

 water. 200 c.c. of the solution are raised to boiling point in 

 a capsule, and 25 c.c. of Liquor ferri sesquichlorati P. Of. are 

 added, and the whole is boiled, with stirring, for two to five 

 minutes more. A precipitate is formed. After cooling the 

 liquid is filtered, and the precipitate Avhich remains on the 



