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acetate and ammonium carbonate., This species also develops best on broth agar at 

 30" to 35 C. 



For beginners it must be noted that on plate Ka the facultative anaerobes, such 

 as Bacterium aerogenes and B. coli, develop quite well, as may be proved by streaking 

 off all the colonies Ak on aerobic plates on which the anaerobes only do not grow. 

 This is in accordance with the fact that at the starting of the experiment some oxygen 

 is present in Lr sufficient for the very small oxygen want of the facultative, better 

 called temporary anaerobes. 



