;,h CHAPTER V. 



(The mixture does not keep long, forming ethyl acetate, 

 which precipitates.) 



Isolated "vn of AscanSj even though furnished with a 

 shell, a iv iixed in twenty-five to thirty seconds. Entire 

 oviducts take about ten minutes. The liquid is therefore 

 one of the most penetrating and rapidly acting of any, if not 

 tin ni"-t. 



\Va-h out with alcohol until all traces of odour of the 

 acetic acid have disappeared (I myself wash out with alcohol 

 containing tincture of iodine). I consider this a very fine 

 reagent. 



Ohlmacher's mixture see 65. 



's MIXTURE (liicerclie Lab. An at. Roma, iii, 1893, p. 47). 

 T\\> Y"!S. saturated aqueous solution of sublimate, one of absolute 

 ;i]r.,hol, ainl one of glacial acetic acid. 



87. Trichlor-acetic Acid (HOLMGREN, Anat. Hefte, xviii, 1901, H. 

 " per cent, solution in water. Fix (nerve-cells) for 8 to 24 hours, 



\v;isli (nit with alcohol. See also HEIDENHAIN, Zei.t. wiss. Mikr., xxii, 

 l!'i>~'. p. :!_'!. and xxv, 1909, p. 405, who makes a mixture of 6 per cent, 

 sublimate solution with 2 per cent, of trichlor-acetic and 1 per cent, of 

 acetic acid, which he calls " Subtriessig." 



88. Trichlor-lactic Acid (HOLMGREN, Anat. Anz.,-xx, 1902, p. 435). 



As the last. Gives rise to serious swelling. 



89. Salicylic Acid (HEIDENHAIN, Arch. mik. Anat., Kv, 1899, p. 

 Saturated solution in one-third alcohol. A trial has given me 



-imply atrocious results. 



90. Chloride and Acetate of Copper (Ripart et Petit' s Liquid, 

 CAT:\OY, La Biologie Cellulaire, p. 94). 



Camphor water (not saturated) . 75 grms. 

 Distilled water . . . .75 



Crystallised acetic acid . . .1 gnn. 



Acetate of copper . . . 0'30 



Chloride of copper . . . O30 



This is a very moderate and delicate fixative, extremely 

 useful for objects that are to be studied in as fresh a state as 

 possible in ac, neons media. Objects fixed in it stain instan- 

 taneously and pcrl'ccily with methyl green. Osmic acid 

 ln: '. v '"' : " |(1(1(1 f " the liquid to increase the fixing action. 

 For cytological researches a valuable medium.' 



