FIXING AND HARDENING AGENTS. (37 



HELD (Abk. Sticks. Ges. Wiss., xxxi, 1909, p. 190) tala-s 

 3 per cent. sol. of bichromate with 4 per cent, of fonnol 

 and 5 per cent, of acetic acid (for inner ear). See also 

 MOREL and BASSAL, Juuni. Auat. Phys., xlv, 1909, p. 03-!. 



114. Chromic Acid Formol. Lo BIANCO fixes marine animals 

 for half to one hour in 10 parts of 1 per cent, chromic 

 acid with 1 of formol and 9 of sea- water,, and passes 

 into graded alcohols. 



MARCHOUX (from PEREZ, Arch. Zool. Espur., v, 1910, 

 p. 11) takes 11 parts 1 per cent, chromic acid, 1 of acetic 

 acid, 4 of water, and 16 of formol (added just before 

 using) . 



115. Copper Formol. NELIS (Ball. Acad. Sc. Belg., 1899 [1900], 

 p. 726) fixes spinal ganglia for twenty-four hours in 1 litre of 7 per cent. 

 formol with 5 c.c. of acetic acid, 20 g. of cupric sulphate, and sublimate 

 to saturation. 



STAFFERS (La Cellule, xxv, 1909, p. 356) used (for Syinpoda) a mixture 

 of GTILSON'S : 100 parts of formol of 5 per cent, with 2 of nitrate of 

 copper. 



STRONG (Jo urn. Comp. Neur., xiii, 1903, p. 296) fixes the head of 

 Acanthias by injecting a mixture of equal parts of forniol and 5 per 

 cent, solution of bichromate of copper. 



116. Nitric Acid Formol. WILHELMI (Fautia u. Flora Golf. Ncapel, 

 xxxii, 1909, p. 15) fixes Triclads in APATHY'S mixture of equal parts of 

 6 per cent, nitric -acid and 6 per cent, formol, and brings them direct 

 into strong alcohol. 



117. Acetone Formol. BING and ELLERMANN (Arch. Anat. 

 Phys. Abth., 1901, p. 260) fix medullated nerves in 9 parts of acetone 

 with 1 of formol. 



