ILEMATKIN (lI.^MATOXYUN) STAINS. 163 



washing out with alum solution or a weak acid. The solution keeps 

 admirably . 



RAWITZ (Leiffaden, 2nd ed., p. 63) takes 1 grin, hsematein, 6 grms. 

 ammonia alum, 200 grms. each of water and glycerin. 



Or (Zeit. wiss. Mik., xxv, 1909, p. 391) 1 grm. hsenmtein, 10 grms. 

 of nitrate of aluminium, 250 grms. each of water and glycerin. 



252. HANSEN'S Solution (Zool. Anz., 1895, p. 158). See fourth edition. 

 Hansen oxidises a mixture of alum and hsematoxylin by means of per- 

 manganate of potash. I find it does not keep. See also MAYER in 

 Mitth. Zool. Mat. Neapel, xii, 1896, p. 30:), or the Grundzilge, LEE and 

 MAYER, 1901, p. 171. 



253. HARRIS'S Solution (Micr. Bull., xv, 1898, p. 47 ; Journ. App. 

 Mic., iii, p. 777). Aluni-hsematoxylin solution ripened by addition of 

 mercuric oxide. MAYER (Grundziige, 1901, p. 171) finds the formula 

 " gives too much hsematein." 



254. Bbhmer's Hsematoxylin (Arch. mik. Artat., iv, 1868, p. 345 ; 

 Acrzt. IntelligenzbL, Baiern., 1865, p. 382). Make (A) a solution of 

 hamiatox. cryst. 1 part, alcohol (absolute) 12 parts and (B) alum 1 part, 

 water 240. For staining, add two or three drops of A to a watch-glassful 

 of B. 



The alcoholic solution of haematoxylin ought to be old and dark 

 ( 237). 



255. Delafield's Hsematoxylin (Zeit. wiss. Mik., ii, 1885, p. 

 288 frequently attributed erroneously to GTRKNACHER or 

 PRUDDEN). To 400 c.c. of saturated solution of ammonia- 

 alum (that is about 1 to 11 of water) add 4 grms. of 

 liasmatox. cryst. dissolved in 25 c.c. of strong alcohol. 

 Leave it exposed to the light and air in an unstoppered 

 bottle for three or four days. Filter, and add 100 c.c. of 

 glycerin and 100 c.c. of methylic alcohol (CH 4 0). Allow 

 the solution to stand (uncorked) until the colour is sufficiently 

 dark, then filter. 



This solution keeps for years. It is well to allow it to 

 ripen for at least two months before using it. 



For staining, enough of the solution should be added to 

 pure water to make a very dilute stain. It is an extremely 

 powerful stain. 



It is still much used. I find that when well rimmed for 

 years rather than months it is quite a first-class stain. 



BUTSCHLI (Unters. iib. mikroscopisclie Schiiumc u, ds Protoplasma, 



