194 CHAPTER XV. 



of one part of methylen blue, and one of potassium carbonate in 100 of 

 water, diluted with about seven parts of water. After forty minutes 

 they are flooded (not washed) with water, and differentiated for about 

 5 minutes in alcohol of 95 per cent. Absolute alcohol, xylol, balsam. 



315. Other Eosin and Methylen -blue Stains. For some very 

 important ones see under " Blood." 



316. Light Green (Lichtgriin S. F.). An " acid " colour, 

 soluble in water or alcohol,, and a good plasma stain. 



BENDA (Verh. physiol. G-es. Berlin, Dec. 18th, 1891, Nos. 

 4 u. 5) stains sections for twenty-four hours in anilin-water 

 safranin solution, then for about half a minute in a solution 

 of 0*5 grm. Lichtgriin or Saureviolett (Grubler) in 200 c.c. 

 of alcohol, dehydrates and mounts in balsam. This process 

 gives a very elegant stain, but requires very thin sections, 

 and there is always risk of the safranin being washed out. 

 The Lichtgriin stain unfortunately does not keep at all well. 



See also PBENANT, Arch. mik. Anat., vii, 1905, p. 430, and 

 GITJEYSSE, C.R. Sue. Bwl, Ixii, 1907, p. 1212. 



317. Janus Green (MICHAELIS, Arch. mik. Anat., Iv, 1900, p. 565). 

 Used in solution of 1 : 30.000 for staining certain granules (pancreas, 

 salivary glands, etc.) in the fresh state. 



318. Malachite Green (syn. Solid Green, Victoria Green, New 

 Green, Benzoyl Green, Fast Green). A basic colour, which has been 

 used as a plasma stain for the ova of Ascaris by VAN BENEDEN and 

 NEYT. These authors used it for glycerin preparations ; it can hardly 

 be got into balsam. 



FLEMMING (Arch. milt. Anat. xix, 1881, p. 324) attributes to it a 

 special affinity for nucleoli. 



319. Iodine Grean (" HOFMANN'S Grim "), see GRIESBACH (Zool. 

 Anz., No. 117, vol. v, 1882, p. 406). Stain essentially that of methyl 

 green, but plasma often violet through the presence of a violet impurity 

 (MA.YER, Mitth. Zool. Stat. Neapel, xii, 1896, p. 311 ; see also earlier 

 editions). It is now only used by botanists. 



320. Thiophen Green (Thiophengrun), see KRATJSE, Intern. Mon- 

 . Anat., etc., iv, 1887, Heft 2. 



321. Coerulein S., a green "acid" dye, is recommended for the 

 staining of muscle-fibrils by M. v. LENHOSSEK (Anat. Anz., xvi, 1899, p. 

 339). See also HEIDENHAIN, Hid., xx, 1901, p. 37, and RAWITZ. ibid.. 

 xxi, 1902, p. 554. 



