1'LASMA STAINS WITH COAL-TAU DYES. 195 



322. Quinolein Blue (Cyanin, Chinolinblau ; v. RANVIER, Trait*', 

 p. 102). Quinolein is said by Ranvier to Lave the property of staining 

 fatty matters an intense blue. 



It is useful for staining Infusoria, which in dilute solution it stains 

 during life. See the methods of CERTES. 



From the reactions mentioned by Ranvier it would seem that his " bleu 

 de quinolcme " is not the preparation that usually goes under that name. 

 See EHRLICH, in Arch. mik. Anat., xiii, 1877, p. 266. 



323. Indulin and Nigrosin. Indulin, Nigrosin, Indigen, 

 Coupier's Blue, Fast Blue R, Fast Blue B, Blackley Blue, Guernsey 

 Blue, Indigo substitute are the names of brands of a group of dyes, 

 mostly " acid/' related to the base violanilm. According to BEHRENS 

 the name Indulin is generally given to a bluish brand, and that of 

 Nigrosin to a blacker one. 



Nigrosin, used with sublimate material, I find stains both nuclei and 

 cytoplasm, the chromatin strongly. It will not give the stain at all 

 with chrom-osmium material. 



According to CALBERLA (Morpli. Jahrb., iii, 1877, p. 627) the concen- 

 trated-aqueous solution of Indulin should be diluted with six volumes 

 of water. Sections will stain in the dilute solution in five to twenty 

 minutes. He also says that it never stains nuclei ; the remaining cell- 

 contents and intercellular substance are stained blue. This seems to 

 me to be, roughly, correct. 



324. Safranin and Nigrosin (or Indigo-Carmine) (KossiNSKi, 



Zeit. wiss. Mik., vi, 1880, p. 61). See early editions. 



325. Picro-Nigrosin, PFITZKR (Deutscli.Botau. Geselhch., 1883, 

 p. 44) dissolves nigrosin in a saturated solution of picric acid 

 in water, and uses it for fixing and staining at the same 

 time, on the slide. See also under " Connective Tissues." 



326. Anilin Blue. Under this title are comprised various 

 " basic '' derivatives of the base rosanilin. They occur under 

 the names Spirit Soluble Blue (Bleu Alcool), Gentian Plue 6 B, 

 Spirit Blue 0, Opal Blue, Bleu de Nuit, Blue Lumiere, Parma 

 Bleu, Blue de Lyon. Some authors give the name Bleu de 

 Nuit and Griindstichblau as synonyms of Bleu de Lyon. The 

 Encycl. mik. Technik. says it is " Anilinblan B 6 B," with 

 many synonyms, or designations of brands, Parma blue being 

 "Anilinblau R or 2 R." I find it a fairly good stain, giving 

 very good differentiations of nerve-tissue and of cartilage 

 (as has already been pointed out by BAUM GARTEN and by 

 JACOBY). MAURICE and SCHULGIN stain in bulk with it 

 after borax-carmine, using a very dilute alcoholic solution. 



