232 CHAPTER XVIII. 



(Verh. Berl. Phys. Gses., 1887); GIERKE (Zeit. wins. Mik. } i, 

 1884, p. 505). 



Sections should be very well washed before being passed 

 from eosin into hrematoxylin or the reverse, as eosin very 

 easily precipitates hsematoxylin. 



For the complicated and superfluous mixtures of RENAUT 

 and of EVERARD, DEMOOR and MASSART, see Foi/s Lehrbuch, 

 p. 196 ; Ann. Inst. Pasteur, vii, 1893, p. 166, or early 

 editions. 



394. Haematoxylin and Congo.- -See 305. 



395. Haematoxylin and Safranin. BABL (Morph. Jahrb., x, 1884, 

 p. 215) stained very lightly with very dilute DELA.FIELD'S haematoxylin for 

 twenty-four hours, then for some hours in (PFITZNER'S) safranin and 

 washed out with pure alcohol. The plasma stain is here given hy the 

 ha3matoxylin. 



Similarly REGAUD, Verh. Anat. Ges., xiv, 1900, p. 112. 



FOA (Festschr. Virchow, 1891, p. 481) stains in a mixture 

 of 25 c.c. of Bohmer's hsematoxylin, 20 of 1 per cent, solution 

 of safranin, and 100 of water for one to three minutes. 



396. Haematoxylin and Saurefuchsin. Stain first with iron 

 hsematoxylin or haemalum, then stain (sections) in 0'5 per 

 cent, aqueous solution of Saurefuchsin, dehydrate and 

 mount. 



397. Haematoxylin and Saurefuchsin and Orange. Proceed 

 as above, using for the second stain the following mixture : 

 Saurefuchsin, 1 grin. : orange, 6 grins. ; rectified spirit, 

 60 c.c. ; water, 240 c.c. (from SQUIRE'S Methods and Formula?, 

 p. 42). Using orange Of. (not mentioned by SQUIRE), I have 

 had very good results. 



The method of CAVAZZANI (Riforma Med., Napoli, 1893, 

 p. 604 ; Zeit. wiss. Mik., xi, 3, 1894, p. 344) is far too com- 

 plicated. 



398. Haematoxylin and Picro-Saurefuchsin (VAN GIBSON, New 

 York Med. Journ., 1889, p. 57 ; quoted from MOELLER, Zeit. 

 wiss. Mik., xv, 2, 1898, p. 172, which see for further details). 

 Proceed as above, using for the second stain the picro- 



