272 CHAPTER XXIII. 



For vibratile epithelium RAN VIE R finds the mixture in- 

 ferior to one-third alcohol. 



526. Sodium Chloride and Formaldehyde. G-AGE recommends 

 the addition of 2 parts of formalin to 1000 parts of normal 

 salt solution (quoted from FISH, Proc. Amer. Mic. Soc., xvii, 

 1895, p. 328). 



527. Caustic Potash, Caustic Soda. These solutions should 

 be employed strong, 35 to 50 per cent. (MOLESCHOTT) ; so em- 

 ployed they do not greatly alter the forms of cells, whilst 

 weak solutions destroy all the elements. (Weak solutions 

 may, however, be employed for dissociating the cells of epi- 

 dermis, hairs, and nails.) The strong solutions may be em- 

 ployed by simply treating the tissues with them on the slide. 

 To make permanent preparations, the alkali should be 

 neutralised by adding acetic acid, which forms with caustic 

 potash acetate of potash, which constitutes a mounting 

 medium (see BEHRENS, KOSSEL, and SCHIEFFERDECKER, Das 

 Mikroskop, i, 1889, p. 156). See also GAGE, Proc. Amer. 

 Soc. of Microscopists, 1889, p. 35. 



528. Baryta- water, Lime-water (FoL, Lehrb., p. 110). Baryta- 

 water will macerate nerve, muscle, and connective tissue in a few hours, 

 lime-water in a few days. 



529. Sulphocyanides of Ammonium and Potassium (STIRLING, 

 Journ. Anat. and Phys., xvii, 1883, p. 208). 10 per cent, solution of 

 either of these salts, for epithelium. Macerate small pieces for twenty- 

 four to forty-eight hours. 



SOULIER (Travaux de Vlnst. Zool, de Montpellier, Nouv. Ser., 2, 1891, 

 p. 171) has found that STIRLING'S solution greatly deteriorates cellular 

 elements, but that good results are obtained by combining it with a 

 fixing agent. The best results were obtained with a 2 per cent, solution 

 of sulphocyanide combined with liquid of RIPART and PETIT ; good 

 ones, by combining liquid of RIPART and PETIT with artificial serum of 

 KRONECKER instead of sulphocyanide, or with pepsin, eau de Javelle, 

 10 per cent, sulphate of soda, or 1'5 per cent, solution of caustic soda ; 

 also by combining solutions of chloride of sodium, or solutions of 

 caustic potash or soda, with any of the usual fixing agents. 



530. LANDOIS'S Solution (Arch. mikr. Anat., 1885, p. 445). 

 Saturated sol. of neutral chromate of ammonia 5 parts. 



Saturated sol. of phosphate of potash . . 5 



Saturated sol. of sulphate of soda . . . 5 



Distilled water . . . . . . 100 



