330 CHAPTER XXVI. 



See also some modifications by KOLSTER, Bcitr. path. Anat., li, 1911, 

 p. 209, consisting in fixation and mordanting in certain chrome alum 

 and chromium fluoride mixtures. 



DUESBERG (loc. cit. ante) has found that the treatment with the 

 chromic and pyroligneous acid and bichromate may be suppressed 

 with advantage. 



SZUTS employs the aluminium-alizarin stain given 335, in lieu of 

 the iron-alizarin for Benda's process. 



Some workers (so MEVES) prefer to harden as BENDA, but 

 to stain with iron hsematoxylin instead of by the alizarin 

 process ; the special hardening rendering the hsematoxylin 

 stain sufficiently specific. Thus also DINGIER, Arch. Zell- 

 forsch., iv, 1910, p. 673. 



ARNOLD (ibid., viii, 1912, p. 256) stains first with iron 

 haematoxylin, differentiates, stains for twenty to thirty min- 

 utes with saturated aqueous solution of thionin, passes up 

 to absolute alcohol, stains for two minutes with Orange Gr. 

 dissolved in clove oil, and passes through xylol into balsam. 

 Chromatin blue, chondriosomes black. 



PENSA (ibid. p. 612) has studied the mitochondria and chloroplasts in 

 plant cells by RAMON Y CAJAL'S silver method for neurofibrils, applied 

 to sections of fresh tissues (a few minutes to an hour in silver of 1 to 

 2 per cent., reduction for ten minutes to an hour in a hydroquinoii 

 bath). 



RENAUT (Comptes rend.,clii, 1911, p. 536) demonstrates mitochondria 

 in fresh cartilage cells by mounting sections in a mixture of artificial 

 serum and saturated aqueous solution of methyl violet 5 B. 



