338 CHAPTER XXVIII. 



salt solution,, and examined pieces of muscle in serum of the 

 animal, afterwards fixing with picrate of ammonia and 

 mounting in glycerin jelly. 



The procedure of DOGIEL has been give^n, 342. 



669. Nerve-endings the Gold Method. FISCHER (An-h. mik. 

 Anat., 1876, p. 365) used the method of LOWIT. 



BIEDEBMANN (last section) recommends for Astacus a 

 similar procedure, the preliminary treatment with formic acid 

 being* omitted, and the muscles being put for a couple of days 

 into glycerin after reduction in the acid. 



EANVIER (Traite, p. 813) finds that for the study of the 

 motor terminations of Vertebrates the best method is his 

 lemon-juice process ( 366). 



See also the methods of APATHY, 368, 371. 



670. Nerve-endings the Silver Method. RAN VIE u employs 

 it as follows (ibid., p. 810) : Portions of muscle (gastro- 

 cnemius of frog) having been very carefully teased out in 

 fresh serum, are treated for ten or twenty seconds with nitrate 

 of silver solution of 2 to 3 per 1000, and exposed to bright 

 light (direct sunlight is best) in distilled water. As soon 

 as they have become black or brown they are brought into 1 

 per cent, acetic acid, where they remain until they have 

 swelled up to their normal dimensions. They are then ex- 

 amined in a mixture of equal parts of glycerin and water. 



This process gives negative images, the muscular substance 

 being stained brown, and the nervous arborescence unstained. 

 The gold process gives positive images, the nervous structures 

 being stained dark violet. 



671. Nerve-endings the Bichromate of Silver Method. The 

 rapid method of GOLGI has been used by RAMON Y CAJAL 

 for the terminations of nerves and tracheae in the muscles 

 of insects. See Zeit. iciss. Mik., vii, 1890, p. 332, or fourth 

 edition. A modification is used by WUNDEREE, Arch. mik. 

 Anat., Ixxi, 1908, p. 523. 



672. Muscle-spindles. See CILJMBARIS, Arch. mik. Anat., 

 Ixxv, 1910, p. 692. Principally intra vitam methylen blue, 



