MLOOD AND GLANDS. 361 



minutes runs in osmie acid of 1 per cent.,, and after five 

 minutes more removes the cover. 



DEKHUYSEN (Anat. Anz., xix, 1901, p. 536) recommends a 

 mixture of either 3 or 9 vols. of 2 per cent, osmic acid with 

 1 of 6 per cent, acetic acid, containing ^ per cent, of methylen 

 blue, which he calls " Osmacet/' 



The mercurial liquids of Pacini ( 414) used to be con- 

 sidered good. HAYEM (" Du Sang" etc., Paris, 1889; see 

 also Zeit. wis*. Mik., vi, 1889, p. 335) has the following 

 formula : sublimate 0'5, salt 1, sulphate of soda 5, and water 

 200. This should be mixed with blood in the proportion of 

 about 1 : 100. Eosin may be added to it. Lo WIT'S formula 

 (Sitzb. k. Akad. Wiss. Wien, xcv, 1887, p. 144) consists of 

 5 c.c. cold saturated sublimate solution, 5 grins, sulphate of 

 soda, 2 grms. salt, and 300 c.c. water. Mosso finds that both 

 of these are too weak in sublimate. 



DUBOSCQ (Arch. Zool. Exper., vi, 1899, p. 481) uses (for 

 blood of Chilopoda) a solution of acetic acid, copper acetate, 

 copper chloride, osmic acid, thionin, 1 grm. each, water 400, 

 which, mixed with the blood, fixes and stains in about two 

 minutes. 



Formol has lately been used. MARCANO (Arch, de Med. 

 Exper., xi, 1899, p. 434) mixes fresh blood with a mixture of 

 100 parts of sodium sulphate of sp. gr. 1*020 and 1 of forrnol ; 

 or with water 85 to 100 parts, sodium chloride ], and 



formol 1. 



KIZER (Journ. Roy. Mic. Soc., 1900, p. 128) simply mixes 

 1 drop of blood with 3 of 2 per cent, formalin, and allows to 

 stand for an hour. 



SCHRIDDE (Uxmat. Tt'chii. Jena, 1910, p. 17) lets blood drop 

 into a mixture of I part of formol, 9 of liquid of Miiller, and 

 10 of water, fixes therein for 2 to 4 hours at 40 C., filters, 

 washes and brings through alcohol and chloroform into 

 paraffin for sectioning. 



719. Fixing and Preserving in films. MUIR (Journ. of Anat. 

 and Phys., xxvi, 1892) makes cover-glass films and drops 

 them into saturated sublimate solution, and after half an hour 

 washes, dehydrates, and passes through xylol into balsam. 



GULLAND (Brit. Ned. Jouin., March 13th, 1897; Scottish 

 Med. Journ., April, 1899) makes cover-glass films, and after 



