362 CHAPTER XXX. 



a few seconds drops them face downwards into a solution 

 of 



Absolute alcohol saturated with eosin . 25 c.c. 

 Pure ether . . . . 25 



Sublimate in absolute alcohol (2 grins. 



to 10 c.c.) . . . . . 5 drops. 



After three or four minutes they are washed, stained, and 

 mounted in balsam. 



For JUNNKK'S fixing and staining and staining method, see 

 next . 



Many recent authors fix wet films with formol. BENARIO 

 (Dent. med. Wochenschr.j 1895, p. 572) mixes 1 part of 10 per 

 cent, formol with 9 of alcohol (the mixture must be freshly 

 prepared), and plunges films into it for a minute. 



Similarly GUIXAND, with \ part of formol to 9 of alcohol. 



Similarly WEKMEL (see Zeit. irixs. J\lik. } xvi, 1899, p. 50), 

 who combines various stains (methylen blue, eosin, gentian, 

 etc.) with the formol. 



EDINGTON (Brit. Med. Jonrn., 1900, p. 19) exposes films 

 for J5 to 30 minutes to ra-pour of formol under a bell-jar. 



SCOTT (Juurn. of Path, and Bacter., vii, 1900, p. 131) 

 exposes films to the vapour for about 5 seconds and drops 

 into absolute alcohol, and after 15 minutes stains and mounts. 



A short exposure (30 seconds) to vapour of osmium has 

 also been recommended. 



720. Stains for Blood. Fre^h (unfixed) blood can be stained 

 on the slide. 



TOISON (Jovrn. Sci. nn'd. de Lille, fev., 1885; Zeit. iviss. 

 Mil'., 1885, p. 398) recommends that it be mixed with the 

 following fluid : 



Distilled water . . .160 c.c. 



Glycerin (neutral, 30 Baume) 30 



Pure sulphate of sodium . . 8 grammes. 



Pure chloride of sodium . . 1 gramme. 



Methyl violet 5 B . . 0'25 



(The methyl violet is to be dissolved in the glycerin with 

 one half of the water added to it; the two salts are to be 

 dissolved in the other half of the water, and the two solu- 

 tions are to be mixed and filtered.) This mixture stains 

 leucocytes sharply, which facilitates enumeration. 



