BLOOD AND GLANDS. 36 



1-7 



neutral balsam. The result is not a Romaiiowsky stain, but a pale 

 methylen-blue-eosin stain. 



See also WRIGHT, Pub. Massachusetts Gen. Hosp., iii, 1910, p. 1, or 

 Jow-n. R. Micr. Soc., 1910, p. 783. 



For the special technique of eosinophilous cells see MARTINOTTI in 

 Zeit. wiss. Mikr., xxvi, 1909, p. 4 (alphabetical bibliography of eight 

 pages.) 



722. Demonstration of Blood-plates of Bizzozero (KEMP, Studies 

 from the Biol. Lab. Julms Hopkins Univ., May, 1886, iii, No. 6 ; 

 Nature, 1886, p. 132). A somewhat large drop of blood is 

 placed on a slide, and quickly washed with a small stream of 

 normal salt solution. The blood-plates are not washed away, 

 because they have the property of adhering to glass. They 

 may be stained with solution of 0'02 per cent, of methyl 

 violet or 1 : 3000 of gentian violet, in salt solution. To 

 make permanent preparations of them, they should first be 

 fixed, by putting a drop of osmic acid solution on the finger 

 before pricking it. 



They may also be stained in films, especially by the 

 Romanowsky method. According to PAPPENHEIM (" Farb- 

 chemie," p. 107) Wasserblau is almost specific for them. 



WRIGHT (Journ. Morph., xxi, 1910, p. 274) studies them in 

 tissues, after fixation with formol or sublimate (not Zonker) 

 by staining with a modified Giemsa stain, and bringing 

 through acetone and oil of turpentine into turpentine 

 colophonium. Details loc. cit. or Journ. Roy. mic. 8<>c., 

 1910, p. 78:1 



See also DEKHUYSEN, An at. An?.., xix, ll'Ol. p. 533 ; 

 KOPSCLI, Intern. MonatscJir. Anat. Phys, xxi, 1004, p. 3i4, 

 and xxiii, 1906, p. 359 ; DEETJEN, Zeit. phys. Cliem., Ixiii, 

 1909, p. 1. 



723. WEIGHT'S Fibrin Stain (Fortschr. d. Med., v, 1887, 

 No. 8, p. 228). Sections (alcohol material) are stained in a 

 saturated solution of gentian or methyl violet in anilin water 

 ( 286). They are brought on to a slide and mopped up 

 with blotting-paper, and a little Lugol's solution is poured 

 on to them. After this has been allowed to act for a sufficient 

 time they are mopped up with blotting-paper, and a drop of 

 anilin is poured on to them. The anilin soon becomes dark, 

 and is then changed for fresh once or twice. The anilin is 



