BLOOD AND GLANDS. 369 



KULTSCHIZKY (Arch. mik. Anat., xlix, 1897, p. 8) fixes in 

 his mixture ( 57), and stains sections either in safranin with 

 2 per cent, acetic acid,, or in a similar solution of neutral red 

 (two to three days, washing out with alcohol). 



MAYER (Mitt. Zuol. Stat. Neapel., xii, 1896, p. 303, or last edition) gives 

 the following two formulae for mixtures that stain exclusively mucus. 



725. MAYER'S Muci carmine (op. cit., last ). One gramme of car- 

 mine, and 0'5 grni. of aluminium chloride with 2 c.c. of distilled water 

 heated over a small flame for two minutes, and made up to 100 c.c. with 

 50 per cent, alcohol. This gives a stock solution, which is as a rule to 

 be diluted for use tenfold with distilled or tap water. 



726. MAYER'S Muchaematein (ibid.). Hsematein 0'2 grm., alu- 

 minium chloride O'l grm. glycerin 40 c.c., water 60 c.c. An alcoholic 

 solution may be made by dissolving in 100 c.c. of 70 per cent, alcohol, 

 with or without the addition of two drops of nitric acid. 



v 



727. Mucicarminic Acid (RAWITZ, Anat. Anz., xv, 1899, p. 439). 



One gramme of carminic acid, 2 of aluminium chloride, and 100 c.c., of 

 50 per cent, alcohol. 



728. Goblet Cells. So far as these contain mucin they give 

 the reactions above described, see PANETH, Arch. mik. Anat., 

 xxxi, 1888, p. 113 ct seq. ; LIST, ibid., xxvii, 1886, p. 481 ; 

 and GUYEISSE, C. R. Soc. BioL, 1907, p. 1212. 



For intestinal epithelium, especially the cells of PANETH, 

 see also MARTIN, Unters. uel>. Oberfldchen n. Driisenepitliel, 

 Leipzig, 1910; and KULL, Arch. mik. Anat., Ixxvii, 1911, p. 

 541 (sections stained with alum ha3inatoxylin, treated for 20 

 to 30 seconds with tincture of iodine, stained a few minutes 

 with Victoria blue, then with eosin). 



729. Salivary Glands. SOLGER (Untertt. z. Naturlehre d. 

 Menschen, xv, 5 and 6, pp. 2- -15; Festschr. f, Geyenbanr, 

 ii, 1896, p. 211) demonstrates the granules in serous cells 

 and half-moons of the submaxillary gland by hardening in 

 a 10 per cent, solution of formol, and then making sections 

 and staining with hsematoxylin of Delafield or of Ehrlich, 

 the granules taking the stain. 



KRAUSE (Arch. mik. Anat., xlv, 1895 p. 94) stains sections 

 either with Heidenhain's iron hsema-toxylin or with Ehrlich- 

 Biondi mixture or tliionin. See also KRAUSE, ibid., xlix, 

 1897, p. 709; and MULLER, Zeit. wis*. ZooL, 1898, p. 640. 



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