NERVOUS SYSTEM CYTOL()(JLOAL METHODS. 387 



10 parts of anilin oil with 90 parts of 90 per cent, alcohol, 

 and as soon as no more colour is given off from them are gut 

 on to a slide, dried with filter-paper, cleared with oil of 

 cajeput, dried again with filter-paper, treated with a few 

 drops of henzin, and mounted. 



VAN GEHUCHTEN (in litt.) prefers to take paraffin section*, 

 mounted on slides by the water method, and stain them for 

 five or six hours in NissFs mixture in a stove kept at 35 to 

 40 0. 



REHM (Hunch, med. Wochenschr., 1892, No. 13 ; Zeit. wiss. 

 Mik., ix, 1893, p. 387) stains for half a minute to a minute 

 in a hot 0*1 per cent, of methylen blue, washes in 96 per cent, 

 alcohol till no more colour conies away, clears with origanum 

 oil, and mounts in balsam. 



GOTHARD (C. R. Soc. BioL, v, 1898, p. 530) stains cel- 

 loiilin sections for twenty-four hours, without heat, in Unna's 

 polychromatic methylen blue and differentiates in a mixture 

 of 5 parts of creosote, 4 of oil of cajeput, 5 of xylol, and 16 

 of absolute alcohol. 



LUITHLEN and SORGO (Nenrol. Centralb , xvii, 1898, p. 640) 

 differentiate in Unna's glycerin-ether mixture ( 702), 

 remove this with absolute alcohol, and clear in origanum 

 oil. 



LENNHOFF (ibiJ., 1910, p. 1) recommends polychrome 

 methylen blue for 2 minutes, followed by Griibler's " Karbol- 

 Methylgrun-Pyronin ; for 20 minutes. 



LORD (Journ. Ment. Sci., October, 1898) makes sections of 

 fresh tissue, frozen, treats them for a few seconds with a 

 mixture of equal parts of 6 per cent, formaldehyde and 

 saturated solution of picric acid, then rinses, and warms till 

 bubbles appear in 5 per cent, solution of methylen blue. 



MENTZ VON KROGH (Centralb. Bakt.,\vm, 19J1, p. 95) stains 

 paraffin sections for 5 minutes in polychrome methylen blue, 

 treats for 1 to 15 with 2 per cent, chromic acid, differentiates 

 till blue with 5 per cent, tannin, and mounts in balsam. 

 Shows also axis cylinders. 



See also GOLDSCHEIDER & FLATAU, Normale und. path. Anat. der 

 Nervenzellen, etc., Berlin, Kornfeld, 1898 (Zeit. wiss. Mile., xvi, 1899, 

 p. 102), and NISSL'S remarks thereon, Deutsche Zeit. Nervenheilk., xiii, 

 1899, p. 318 (Zeit. wiss. Mik., xvi, 1899, p. 370) ; Cox, Inttrn. Monatsschr. 

 Anat. Phys., xv, 1898, Heft 8 ; MYERS, Anat. Record, 1908, p. 434 ; 



