388 CHAPTER XXXI J. 



ILBERG, Neurol. Centralb., 1896, No. 18 ; SAVINI, Centralb. Bakt.. 1909, 

 p. 697. 



759. Methylen Blue and Erythrosin. HELD (Anat. Phys.jAnat. 

 Abth. } 1895, 1896, p. 399) stains sections on slides, with the; 

 aid of a gentle heat, for one or two minutes in a solution of 

 1 grin, of Griibler's erythrosin in 150 of water with two 

 drops of glacial acetic acid ; washes out with water, and 

 stains in a mixture of equal parts of Nissl's methylen. blue 

 and 5 per cent solution of acetone, warming strongly the 

 while, until all odour of acetone has disappeared. After 

 cooling he differentiates with O'l per cent, solution of alum 

 until the sections appear reddish, rinses in water, dehydrates 

 as rapidly as possible in absolute alcohol, and passes through 

 xylol into balsam. See further hereon HELD, op. cit., 1897, 

 pp. 226 233, 273 305 (Supplementband), and BOCCARDI, 

 Man. Zuol. ItaL, x, 1899, p. 141 (stains in a mixture of 

 erythrosin O'l, toluidin blue, 0'2, and water 100 parts, and 

 differentiates in 0*5 per cent, alum solution). 



760. Thionin. LENHOSSEK (Fein. Ban. d. Nerveiisy stems, 

 Berlin, 1894, p. 149) stains sections of formal material for 

 five minutes in a concentrated aqueous solution of thionin, 

 rinses with water, and mounts as Nissl. The stain does not 

 keep well. 



Similarly RAMON Y CAJAL, Man. de Anat. Path. Gen., 1896 

 (see Zeit. wiss Mik., xv, 1899, p. 375), and LUXENBURG, 

 Neurol. Centralb., xviii, 1899, p. 629. 



761. Toluidin Blue. LENHOSSEK (Neurol. Centralb., xvii, 

 1898, p. 577 ; Zeit. ivixs. Mik., xv, 1899, p. 492). Sections 

 are stained on slides for a night in concentrated solution of 

 toluidin blue, rinsed in water, quickly differentiated with 

 alcohol, cleared with xylol or carbolic-acid xylol, and mounted 

 in balsam. 



Similarly POLUMORDWINOW (Zeit. wixs. Mik., xvi, 1899, 

 p. 371, who stains in a very weak alkaline solution, 1 part of 

 1 per cent, solution to 119 of water and 1 of carbonate of soda, 



762. Neutral Red. JULIUSBURGER (Neurol. Centralb., xvi, 

 1897, p. 2f;9) stains sections of formol material, for half to 

 three quarters of a minute in n:arm 1 per cent, solution of 



