404 CHAPTER XXXTI. 







hydroquinon, 5 grins, sodium sulphite, 50 c.c. formol of 20 per 

 cent., and 1000 c.c. water. Wash and imbed, preferably in 

 celloidin. Tone the sections until grey in 1000 c.c. water 

 with 30 grins, each of hyposulphite of soda and sulphocyanide 

 of ammonium and 10 per cent, of 1 per cent, gold chloride. 

 It is well, though not necessary, to treat them first with a 

 solution of 0'5 grm. of permanganate of potash and 1 grm. of 

 sulphuric acid in 1000 of water, and then with 1 per cent, 

 solution of oxalic acid, before mounting. 



LEGENDRE (Anat. Anz., xxxvi, 1910, p. 209) omits the toning and per- 

 manganate, and imbeds in paraffin. 



Similarly, COLLIN et LUCIEN, Biblioyr. Anat. Supp., 1909, p. 238. 



SARAGNONE (Patologica, i, 1909, p. 536; Jmtrn. Roy. Micr. Soc., 1910, 

 p. 256) silvers with a mixture of 30 c.c. of tacliiol (10 per cent, solution 

 of silver fluoride) with 100 of water. 



BESTA (Anat. Anz., xxxvi, 1910, p. 477) fixes for two days in 20 parts 

 of formol with two of acetic aldehyde and 80 of water, washes for 

 twenty-four hours in distilled water changed 7 or 8 times, and puts for 

 two days into 4 per cent, solution of ammonium molybdate, makes 

 paraffin sections, stains in a 1 : 1000 solution of thionin, differentiates in 

 3 parts of creosote to 1 of absolute alcohol, and passes through pure 

 creosote and xylol into neutral balsam. Recommended for Purkinje 

 cells and spinal ganglia of young subjects. 



KOPSCH (Sitzb. Acad. Wiss. Wien, xl, 1902, p. 929 ; Zeit. wiss. Mik., 

 xx, 1904, p. 347) demonstrates it in spinal ganglion cells as follows; the 

 ganglia are put for eight days (or exceptionally, a few more), into osmic 

 acid of 2 per cent, and paraffin sections made. The network becomes 

 quite black and is sharply demonstrated, unless the impregnation has 

 been too prolonged, in which case the rest of the cell body becomes 

 blackened also. 



SJOVALL (Auat. Hefte, xxx, 1906, p. 362) fixes in formol before treat- 

 ing with the osmic acid. 



778. Medullary Sheath (Nemoceratin, etc.). GOLGI (quoted 

 from REZZONICO, Arcli. per la Sri. mcd., iv, 1870, p. 85) 

 puts pieces of spiral cord into 2 per cent, solution of 

 bichromate of potash, for eight to fifteen days in summer, or 

 a month in winter. Wash, and put into 0'75 per cent, 

 solution of nitrate of silver, for two or three days in summer, 

 or eight or ten or more in winter. Pass through alcohol into 

 oil of turpentine, tease therein, and mount in damar. Expose 

 to sunlight therein for eight to ten days; or to diffused day- 

 light for twenty to forty days. Demonstrates funnels and 

 spirals, 



