412 CHAPTER XXX II I. 



TSCHERNYSCHEW and KARUSiN (Zeit. wiss. Mik., xiii, 1896, p. 354'), 

 stains for twenty four hours in the haBmatoxylin of KULTSCHITZKY, 

 next . 



So also PAVLOW, (Ibid., xxi, 1904, p. 14, taking the permanganate twice 

 as strong as Pal. 



KOZOWSKY (Neurol. Zentralb., xxiii, 1904, p. 1041) stains as Weigert, 

 and differentiates the sections first with 1 per cent, permanganate, till 

 the grey comes out brown, and finishes the differentiation with Liq. 

 ferri sesq uich lorati. 



POTTER (Zeit. wiss. Mik., xxvii, 1910, p. 238) stains as Weigert, last, 

 and differentiates first in permanganate of 0'25 per cent., then in borax 

 ferricyanide. 



784. KAISER (Neurol Ctntralb., xii, 1893, pp. 364, 368; Zeit. wiss. 

 Mik., xi, 1894, p. 249) hardens first in liquid of Miiller, then for eight 

 days in liquid of MARCHI ( 796), mordants sections for five minutes 

 with sesquichloride of iron (1 part to 1 of water and 3 of 70 per cent, 

 alcohol), stains, and differentiates with Pal's liquid. For details see 

 early editions. 



BOLTON (Journ. of Anat. and Pliys., xxxii, 1898, p. 245) makes sections 

 of formalin material, and mordants them for a few minutes in 1 per cent, 

 osmic acid, or for a few hours in iron-alum or ammonium molybdate, 

 stains in KULTSCHITZKY'S ha^matoxylin (next ), and differentiates by 

 Pal's process. 



Similarly WYNN, ibid., 1900, p. 381. 



LASLETT (Lancet, 1898, p. 321 ; Journ. Eoy. Mic. Soc., 1898, p. 600) 

 mordants in liquid of Marchi (1 week), makes sections, stains by KULT- 

 SCHITZKY'S method, and differentiates by PAL'S. 



785. KULTSCHITZKY'S method (Anat. An?.., 1839,, p. 223, and 

 1890, p. 519). Specimens are hardened for one or two 

 mon tli s in solution of Erlicki, imbedded in celloidin or photo- 

 xylin, and cat. Sections are stained for from one to three 

 hours, or as much as twenty-four, in a stain made by adding 

 1 grin, of ha3inatoxylin dissolved in a little alcohol to 100 c.c. 

 of 2 per cent, acetic acid. They are washed out in saturated 

 solution of carbonate of lithia or soda. 



Differentiation is not necessary., but by adding to the car- 

 bonate of lithia solution 10 per cent, of a 1 per cent, solu- 

 tion of red prussiate of potash, and decolorising therein for 

 two or three hours or more, a sharper stain is obtained. 

 After this the sections are well washed in water and mounted 

 in balsam. Myelin dark blue. 



WOLTEKS (Zeit. wiss. Mik., vii, 189J, p. 466) proceeds as 

 Kultschitzky,, except that lie stains at 45 C. for twenty-four 



