450 CHAPTER XXXVI. 



LIST (Faiuia Flora Golf. Neapel, xxvii, 190 2, p. 292) 

 narcotises Mytilidse with 2 per cent. of cocaine in sea water, 

 and (for preservation of cilia) fixes in sea water, with 10 per 

 cent, of formol. 



Lo BIANCO advises that Prosobranchiata, and, amongst 

 the Heteropoda, Atlantida?, be narcotised with 70 per cent, 

 alcohol, 18. For Opisthobranchiata I recommend sudden 

 killing with liquid of Perenyi, or the acetic method, 852. 

 Aplysia may first be narcotised by subcutaneous injection of 

 about 1 c.c. of a 5 to 10 per cent, solution of hydrochlorate 

 of cocaine (ROBERT, Ball. Sclent, de la France, etc., 1890, 

 p. 449 ; Zeit. wiss. mik., ix, 1892, p. 216), or (SCHONLEIN, 

 Zeit. BioL, xxx, 1893, p. 187) 1 c.c. of 4 per cent, solution of 

 Pelletierin. For Lo BIANCO' s various methods see the 

 original, p. 467. 



For Pteropoda in general, liquid of Perenyi. Creseis is a 

 difficult form. Lo BIANCO advises the alcohol method, 18. 

 For the Gymnosomata he narcotises with O'l per cent, chloral 

 hydrate. 



For terrestrial Gastropods see 23 and 26. MARCHI 

 (Arch, inik. Anat., 1867, p. 204) gets rid of the mucus of 

 the integument of Limax, which may be an obstacle to 

 preparation, by putting the living animal into moderately 

 concentrated salt solution, in which it throws off its mucus 

 and dies in a few hours. 



LANG (Anat. Hefte, 1902, p. 84) puts Helix into water with 

 enough chromic acid to make it of a Rhine-wine colour, 

 with an air-tight cover to the vessel, and when the animals 

 are extruded injects into them a quarter to a half of a 

 Pravaz syringe of 1 per cent, cocain, and after five to fifteen 

 minutes dissects and fixes. 



HKYMANS (Bull. Acad. Bely., xxxii, 1896, p. 578) injects 

 ethyl bromide under the skin of Cephalopoda. 



Lo BIANCO uses for fixing them his chromo-acetic acid, 

 No. 1 ( 39), with a double quantity of acetic acid, for 

 twenty-four hours. 



856. Liver of Mollusca. ENRIQUES (Mitth. Zool. Stat. 

 Neapel, xv, 1901, p. 289) fixes the liver of Octopus and 

 Sepia with sublimate. For Aplysia (especially in summer) 

 alcohol, formol, and chromic mixtures are counter-indicated, 



