METHODS FOR INVERTEBRATES. 457 



silver process. MARTIN (G.R. Soc. Philomath., 1893, p. 3) 

 injects them with indigo white (through the body cavity), 

 and puts into hot water from which the air has been expelled 

 by boiling. Tracheae blue. 



872. Brain of Bees. KENYON (Jonrn. Comp. Neurol., vi, 

 1896, p. 137; Jonrn. Roy. Mic. Soc., 1897, p. 80) treats by 

 the GOI.GI process (seldom successful), or hardens in a 

 mixture of one part formol and two of 5 per cent, sulphate 

 of copper, followed by staining in Mallory's phospho-molybdic 

 haematoxylin. 



JONESCU (Jena. Zeit., xlv, 1909, p. Ill) has employed the 

 silver methods of Ramon y Cajal and Bielschowsky and Wolff. 



873. Ventral Cord. FLOYD (Mark. Anniv. vol. 1904, p. 

 355) fixes the gang'lia of Periplaneta for eighty minutes with 



/ fj O -L O / 



vapour of formol, and brings into alcohol. 



See also BINET, Jonrn. Anat. Pliys., xxx, 1894, p. 469. 



874. Eyes of Arthropods. For the methods of LANKESTER 

 and BOURNE (Quart. Journ. Mic. 8ci., 1883, p. 180 : LimulusJ 

 HICKSON (ibid., 1885, p. 243: Mused); PARKER (Bull. ]\Ius. 

 Harvard Coll., xx. 1890, p. 1 ; Zeit. iri*s. Mil:., viii, 1891, 

 p. 82 : Homarus) see early editions. 



PARKER (Mitth. Zool. Stat. Neapel, xii, 1895, p. 1) also 

 applies the methylen blue method to the retina and optic 

 ganglia in Decapods, especially in Astacns. He injects O'l c.c. 

 of a 0'2 per cent, solution into the ventral sinus. After 

 twelve to fifteen hours the animals are killed, the ganglia 

 (jiiickly dissected out, and the stain fixed as described, 344. 



For his method for eyes of Scorpions see 583. 



For the methods of PUKCELL for the eyes of Phalangida see 

 Zeit. wins. Zool., Iviii, 1894, p. 1. He has the following 

 stain. The cephalothorax is removed and brought for 

 twenty minutes into 50 per cent, alcohol warmed to 45 or 

 50 C., and saturated with picric acid. The pigment 

 dissolves in this solution and stains the nuclei and some 

 other parts of the rhabdoms, so that no further stain is 

 required. 



HENNINGS (Zcit. ?ms. Milt., xvii, 1900, p. 326) depigments 

 sections by putting then for ten minutes (Musca) to twelve 



