466 CHAPTER XXXVI. 



warm (50 to 60 C.) 1 per cent, sublimate in 70 per cent, 

 alcohol. 



BETTENDOEF (Zool. Jahrb., Abtli. Morph., x, 1897, p. 308) 

 has had good results with the rapid Grolgi method only on 

 Distoma hepaticum, and prefers methylen blue. 



HA VET (La Cellule, xvii, 1900, p. 353) has also had results 

 with the Golgi method on this form, and also with thionin, 

 (after fixing with sublimate), which demonstrates tigroid 

 substance. 



Cercarise. SCHWAEZE (Zeit. wiss. Zool., xliii, 1886, p. 45) 

 found that the only fixing agent that would preserve the 

 histolosrical detail of these forms was cold saturated sublimate 



o 



solution warmed to 35 40 C. 



For an " indifferent } liquid, HOFMANN (Zool. Jahrb., xii, 

 1899, p. 176) takes 1 part of white of egg in 9 of normal 

 salt solution. 



892. Turbellaria. BEAUN (Zeit. wis*. Mil", iii, 1886, p. 398) 

 gets entire animals (Rhabdocoela) on to a slide, lightly 

 flattens out with a cover, and kills by running in a mixture 

 of three parts of liquid of Lang with one of 1 per cent, 

 osmic acid solution. (BoHMia \ibid. ~\ t commenting on this, 

 says that for some of the tissues, such as muscle and body 

 parenchyma, nitric acid and picro-sulphuric acid are very 

 useful.) Sections may be made by the paraffin method. 



DELAGE (Arch, de Zool. exp., iv, 2, 1886) recommends fixation (of 

 Rhabdoccela Accela) by an osmium-carmine mixture, for which see loc. 

 cit., or by concentrated solution of sulphate of iron. Liquid of Lang was 

 not successful. 



For staining, he recommends either the osmium-carmine or impreg- 

 nation with gold (^ formic acid, two minutes ; 1 per cent, gold chloride, 

 ten minutes ; 2 per cent, formic acid, two or three days in the dark.) 



BOHMIG (Zeit. 10188. Mile., iii, 1886, p. 239) has obtained instructive 

 images with Plagiostomidse fixed with sublimate and stained with the 

 osmium-carmine. 



G-EAFF (Turbellaria Accela, Leipzig, 1891 ; Zeit. wiss. Mik., 

 ix, 1892, p. 76) says that chromo-aceto-osmic acid, followed 

 by haomatoxylin, is good for the skin, but not for the 

 Rhabdites, which in Acoela and Alloiocoela seem to be 

 destroyed by swelling. The same method is also good for 

 the parenchyma of Ampliiclioerus cinereus, Convoluta paradoza 



