METHODS FOR INVERTEBRATES. 4f)7 



and C. sordida. Sublimate is good for Convoluta Roscoffensis. 

 The nervous system may be investigated by the methods of 



For Dendrocoela sublimate solutions, sometimes hot, appear 

 indicated for fixing ; see the mixture of LANG, 64, also 

 CHICHKUPP (Arch. de. BioL, xii, 1892, p. 438). 



ARNOLD (Arch. ZettforscJi., iii, 1909, p. 433) kills Detidro- 

 ctt'lnm in extension (?) with strong liquid of Flemming. 



OESTERGREN narcotises Dendrocoelum with his ether-water, 

 18. 



JAENICHEN (Zeit. wiss. Zool., Ixii, 1896, p. 256) advises 

 for Planaria, eyes epecially, picro-sulphuric acid for an 

 hour or two ; osmic acid is not good, and liquid of Miiller 

 macerates. He stains with borax-carmine, makes sections, 

 and puts them for ten minutes into osmic acid, then for five 

 minutes into pyroligneous acid, on the top of the stove. 

 He macerates the visual rods in a mixture of one part 

 common salt, one of acetic acid, and 100 of water. He 

 bleaches the pigment of the eyes with peroxide of hydrogen. 



WILHKLMI (ibid., Ixxx, 1906, p. 548) throws Triclads into 

 almost boiling mixture of Zenker, and after 10 to 30 minutes 

 removes to water for some hours, and then passes into iodine 

 alcohol. 



Echinvdermata. 



893. Holothurioidea.- -These are difficult to fix on account 

 of their contracting with such violence under the influence 

 of irritating reagents as to expel their viscera through the 

 oral or cloacal aperture. 



VOGT and YUNG (Anat. Comp. Prat., p. 641) sa}^ that 

 Cucumaria Planci (G. doliolum, Marenzeller) is free from 

 this vice ; but they recommend that it be killed with fresh 

 water, or by slow intoxication, 25. 



Synapta may be allowed to die in a mixture of equal 

 parts of sea water and ether or chloroform (S. Lo BIANCO). 



OESTERGREN ( 18) puts Synapta into his ether water, but 

 Dendrochirota first into magnesium sulphate of 1 to 2 per 

 cent., for some hours. 



GEROULD (Bull. Mus. Harvard Coll., xxix, 1896, p. 125) 

 paralyses Caudina with sulphate of magnesia, 24, and fixes 

 with liquid of Perenyi (or sublimate for the ovaries). 



