METHODS FOR INVERTEBRATES. 479 



puts it into chloroform, which dissolves the paraffin and 

 hardens the collodion. 



See also 137, and PRZESMYCKI, loc. cit. 918. 



922. Sphgerozoa. BRANDT (Fauna u. Flora Golf. Neapel, 

 xiii, 1885, p. 7) fixes with chromic acid of 0'5 per cent, to 

 1 per cent, (half an hour to an hour), or with a mixture of 

 equal volumes of sea water and 70 per cent, alcohol with a 

 little tincture of iodine for a quarter to half an hour, or with 

 a 5 to 15 per cent, solution of sublimate in sea water. 



KAKAWAIEW (Zool. .4?i2.,xviii, 1895, p. 286) fixes Aulacantha 

 for 24 hours in equal parts of strong liquid of Flemming 

 and acetic acid, and hardens for several days in pure liquid 

 of Flemming. 



See also Lo BIANCO, 920. 



923. Sporozoa.--WASiuLEWSKi (Sporozoenkunde, Jena, 1896, 

 p. 153) studies them living in their natural medium, or in 

 normal salt solution, or in a medium composed of 20 parts 

 white of egg, 200 of water, and 1 of common salt. He fixes 

 Gregarinfe and Coccidia with osmic acid, sublimate, or picro- 

 sulphuric acid, and Myxosporidia with liquid of Flemming. 



SCHAUDINN (Zool. JJtrb., Abtli. Anat., xiii, 1900, p. 197) 

 fixes Coccidia with a mixture of 2 parts of saturated aqueous 

 sublimate and 1 of absolute alcohol, with, if desired, a trace 

 of acetic acid. 



STKMPELL (Arch. Protistenk., xvi, 1P09, p. 389) fixes cater- 

 pillars infected with Nos<>mu in two parts of saturated 

 sublimate with one of alcohol and a little acetic acid, and 

 stains sections for as much as four days in GIEMSA'S mixture, 

 rinses with alcohol and passes through xylol into balsam. 



LEGER (ibid., iii, 1904, p. 311) fixes cysts for a minute in 

 " acetic sublimate," puts for a minute into absolute alcohol, 

 and stains as a smear with ha^malum or iron hrematoxylin. 



BRARIL (Arch. Zool. Exper., 4, iv, 1905, p. 74) fixes them for 

 twei^^-four hours in a mixture of 1 grin, picric acid, 15 c.c. 

 acetic acid, 60 c.c. formol and 150 c.c. alcohol of 80 per cent., 

 and stains paraffin sections in iron hrematoxylin followed by 

 eosin and orange G., or Lichtgriin and picric acid. 



924. Hsematozoa. GRASSI (Aft. Accad. Lined, iii, 1900, 

 p. 357) demonstrates the Malaria-parasites in the intestine, 



