CHAPTEIi V. 



The addition of sodium chloride allows a stronger solution to be 



btaiiK'd than can be made with pnre water, and also, it is stated, 



nhances the p.-u.-t rati< >n of the sul .limate. But the fixation-precipitates 



29) t'..rm'l by the double salt are (according to SPULER, EncycL in Ik. 

 T- <]< ,<,'!:.. p. 1^7 J-i for the most part soluble in water, thus giving rise to 

 preservation. 



tod (i. e. over 20 per cent.) solution in sea-water is recom- 

 for some marine animals. 

 STOELZNER (Zrif. wise. Mil-r.. xxiii, 1906, p. 25) recommends saturated 

 solution of sublimate in sugar solution of 4A per cent., as isotonic (for 

 warm-blooded animals). 



Liquid of Lang (Zool. Anzeiger, 1878, i, p. 14). For Planar let. 

 Distilled water .... 100. 

 Chloride of sodium . . . 6 to 10. 

 Acetic acid . . . . 6 to 8. 

 Bichloride of mercury . . 3 to 12. 

 (Alum, in some cases . . .) 



65. Alcoholic Solutions. APATHY (Mikrotechnik, p. HI) 

 recommends a solution of 3 to 4 grammes of sublimate 

 and 0'5 gramme sodium chloride in 100 c.c. of 50 per 

 c'LMit. alcohol, for general purposes. 



OHLMACHER (Joum. Exper. Medicine, ii, 6, 1897, p. 671) takes- 

 Absolute alcohol ...... 80 parts. 



Chloroform . . . . . . . 15 ,, 



Glacial acetic acid . . . . . 5 ,, 



Sublimate to saturation (about 20 per cent.). 



" Ordinary pieces " of tissue are sufficiently fixed in fifteen to thirty 

 minutes. Entire human cerebral hemispheres, subdivided by Meynert's 

 section, take eighteen to twenty -four hours. 



For liquids containing a much higher proportion of acetic acid, see 

 Acetic Alcohol. 



66. Aceton Solution. HELD (Arch. Anat. Phys., Anat. Abth., 

 1897, p. 227), fixes nerve-tissue in a 1 per cent, solution of 

 sublimate in 40 per cent, aceton, and washes out through 

 increasingly concentrated grades of aceton. 



67. Phenol Solution. PAPPENHEIM (Arch. Path. Anat., civil, 1899, 

 p. 23) shakes up carbolic acid with aqueous sublimate solution and 

 filters. 



68. Ciaccio (Arch. Ital. Anat. Embr., vi, 1907, p. 486) has an irrational 

 mixture of sublimate, iodine, and formol. 



69. Mercuro-nitric Mixtures. FRENZEL (Arch. mik. Anat., 

 xxvi, 1885, p. 232) recommends a half-satnrnted solution of 



