

 



iir, CIIAITKK v. 



Mic. Soc., 1^'S p. i:2i are in my view too weak, and suffer by the 

 omission <!' tin- acetic acid. 



BiOBBATJX (/;//,/. And., I'-'lo. p. 2:r>; lakes ]"> parts t'ormol, 85 of 

 trichlor-acetic acid >!' > per rent., and picric acid to saturation. 



111. Picro-platinic Formol (M. and P. BOUIN, Bihl. Anat., 



L898, 1. -1, p. 2). 



Platinum chloride, 1 per cent. sol. 20 parts. 



Picric acid, saturated sol. . . 20 



Formol . . . . . 10 



Formic or acetic acid . . . 5 



1 iiud this excellent, but the mixture does not keep more 



than a day or two. 



BOUIN also (Arch. Biol, xvii, 1900, p. 211) simply 



substitutes formol for the osmic acid in HERMANN'S mixture, 



45. 



112. Sublimate Formol (M. and P. BOUIN, 7oc. cit.). A similar 

 mixture, in which sublimate of 1 per cent, is substituted for the plati- 

 num chloride. 



Another formula of the same authors (Arch. Biol., xvii, 1900, p. -11) 

 is 1 part of formol to 3 of saturated aqueous sublimate. Rinse with 

 water and bring into alcohol of 70 per cent. 



SPULER (Eucycl. mile. Technik., 1st ed., p. 1-80) adds to sublimate of 3 

 PIT cent, or more 1 per cent, of glacial acetic acid and 10 per cent, of 

 formol. 



MANN (Verh. Auut. Ges., 1898, p. 39) takes for nerve-cells 2| g. sub- 

 limate. 1 g. picric acid, 5 c.c. formol, and 100 c.c. water, or (Methods, 

 etc., p. 97) for all tissues 2^ g. sublimate, 20 c.c. formol, and 80 c.c. water. 



BRANCA (Journ. Anat. et Phys., xxxv, 1899, p. 767) adds 10 parts of 

 t'ormol and 1 of acetic acid to 60 parts of saturated solution of picric 

 acid in saturated aqueous sublimate. 



NUWAK (Anat. Anz., xx, 1901, p. 244) takes 30 parts of saturated sub- 

 limate, 30 of 1 per cent, chromic acid, 27 of water, 3 of acetic acid, and 

 1<> of formalin. 



113. Formol-Muller.--This is the name given by Oinii 

 (livrl. 1:1 in. lI'oclniNclir., 1896, No. 13) to a mixture of 1 

 part <>!' r.,rmol with 10 of liquid of Mtiller ( Do). It should 

 be freshly nun It- up. Ki\ for three hours in the stove, or 

 twelve at normal U'liipLTature, wash out with running water. 

 .Much used, (.'specially- for nervous tissues. 



M<>i;u,i;i; (/,-if. wi88. Zool. } Lxvi, 1899, p. 85) takes 1 vol. 

 of formol and 4 of 3 per cent, bichromate (for the intestine 



