H^MATEIN (H2BMATOXYLIN) STAINS. 159 



or the mordant becomes turbid, then stains with the hsematoxylin. in 

 the same way. The whole process takes about ten minutes. 



HELD (Arch. Anat. Phys., Anat. Abth., 1897, p. 277) adds to the 

 staining bath a very little of the iron-alum solution until a scarcely 

 perceptible precipitate is produced. A dangerous practice. I find it is 

 not even safe to add a little of an over-used bath (supra). 



FRANCOTTE (Arch. Zool. Exper., vi, 1898, p. 200) mordants with tarlrate 

 of iron, MALLORY (Journ. Exper. Med., v, 1900, p. 15) with chloride. 



243. Iron Haematoxylin (BUTSCHLI, Unters. ilber miliroskopische 



Schdume u. das Protoplasma, etc., 1892, p. 80). Sections treated with a 

 weak brown aqueous solution of ferric acetate, washed with water, and 

 stained in - 5 per cent, aqueous solution of hsematoxylin. A stain of 

 extraordinary intensity, used by Biitschli for sections, 1 /* in thickness, 

 of Protozoa. 



244. Weigert's Iron Haematoxylin Mixture (Zeit. uriss. Mi~k., xxi, 

 1904, p. 1). Mix one part of a 1 per cent, solution of hsematoxylin in 

 alcohol of 96 per cent, with one of a solution containing 4 c.c. of liq. 

 ferri sesquichlor., 1 c.c. of officinal hydrochloric acid (sp. gr. T124) and 

 95 of water. The mixture may be kept for some days (until it begins to 

 smell of ether), but is best used fresh. Stain sections for a few minutes ; 

 110 differentiation is necessary. 



For an earlier process of WEIGERT'S (Allg. Zeit. Psychiatr., 1894, p. 

 245) see last edition. 



MOREL and BASSAL (Journ. Anat. Phys., xlv, 1909, p. 632) stain in 

 balk in "Weigert's mixture with the addition of 1 c.c. of 4 per cent, solu- 

 tion of acetate of copper. 



245. JANSSENS' Iron Haematoxylin ("Hematoxyline noire"; La- 

 Cellule, xiv, 1897, p. 207). A similar mixture to that of DELAFIELD, 

 ferric alum being taken instead of ammonia alum, the rest as in Dela- 

 field's. A progressive stain, nuclear : for yeast cells. 



246. HANSEN'S Iron Hsematoxylin (Zeit. iviss. Mik., xxii, 1905, p. 55). 

 A solution of 10 grms. ferric alum in 150 c.c. water is added to a 



solution of 1'6 grin, hsematoxylin in 75 c.c. water, the mixture heated 

 to boiling-point and cooled without access of air. Filter before use. 

 To get a pure nuclear stain, add dilute sulphuric acid. 



247. Aluminium Hsematein (Alum Haematoxylin) Generalities. 

 -The mordant and dye are generally combined in a single 



staining bath, giving a progressive stain. The stain is in 

 different tones of bine or red according to the composition of 

 the staining solution. Neutral or alkaline solutions give a 

 blue stain ; acid solutions give a red one. In order to get a 

 l)lue stain in preparations that have come ont red through 



