NUCLEAR STAINS WITH GOAL-TAT?, DYES. 181 



The Philadelphia Medical Journal, May 14th, 1808. It much resembles 

 methylen blue. 



METZNER (Nag-el's Handb. Phys., ii, 1907, p. 915) mordants sections, 

 before staining, for three quarters of an hour in iron alum. 



Magdila Red (JNTaplithalhi Red, Rose de Naphthaline). 



Fuchsin (meaning the basic fuchsins, a series of Rosanilin salts 

 having very similar reactions, and found in commerce under the names 

 of FUCHSIN, ANILIN RED, RUBIN, ROSEIN, MAGENTA, SOLFERINO, 

 CORALLIN). GRASER (D^utsch3 Zeit. Chirurgie, xxvii, 1888, pp. 533 

 584; Zeit. wiss. Mik., v, 1883, p. 378) stains for twelve to twenty-four 

 hours in a dilute aqueous solution, washes out for a short time in alcohol, 

 stains for a few minutes in aqueous solution of methylen blue, and 

 dehydrates with alcohol. A double stain. Chromatin and nucleoli red, 

 all the rest blue. 



ZIEHL'S Carbolic Fuchsin (Zeit. wiss. Mik., vii, 1890, p. 39) 

 consists of fuchsin 1 grin., acid, carbol. crist. 5 grins., 

 alcohol 10 grins., aq. dest. 100 grins. The stain is 

 differentiated with alcohol followed by clove oil. 



Kres, fuchsin (ROTHIG, Arch, mik. Anat., Ivi, 1900, p. 354). Its 

 aqueous solution is red, and stains mucus, cartilage, keratin, and nuclei 

 red, whilst its alcoholic solution is blue and stains elastiii blue. See 

 also under " Connective tissues." 



Bismarck Brown has this advantage, that being sufficiently resistant 

 to alcohol it may be utilised for staining entire objects. 



KAISER (Bill loth. Zool, H. 7, 1 Halft, 1891 ; Zeit. wiss. Mik., viii, 1891, 

 p. 363) stains for forty-eight hours, and at a temperature of 60 C. in 

 saturated solution of Bismarck brown in 60 per cent, alcohol (the solution 

 to be made in boiling alcohol), and washes out (until all is decoloured 

 except the karyokinetic figures) in 60 per cent, alcohol, containing 2 per 

 cent, hydrochloric acid or 3 per cent, acetic acid. 



Methyl Violet. See ante, 278 



Benzoazurin (MARTIN, Zeit. wiss. Mik., vi, 3, 1889, p. 193). Stain for 

 an hour or so in dilute aqueous solution, and wash out withHCl alcohol. 



Methylen Blue. 



Nigrosin (ERRERA. Proc.-Verb. Soc. Beige deMik., 1881, p. 134) gives 

 a good stain which resists alcohol well. 



Methyl Green is sometimes useful in certain mixtures (see next 

 chapter) . 



