OTHER STAINS AND COMBINATIONS. 231 



The solutions of both these colours should be extremely 

 dilute for sublimate material, but strong for chrom- osmium 

 material. It is possible to use them for staining in bulk. 



BAUMGAETEN (Arch, mik. Anat. } xl, 1892, p. 512) stains 

 sections (of material previously stained in borax-carmine) for 

 twelve hours in a 0'2 per cent, solution of bleu de Lyon in 

 absolute alcohol, and washes out for about half that time 

 before mounting in balsam. He recommends the process 

 for cartilage and nerve-centres. 



389. Carmine and Malachite Green. MAAS (Zeit. wiss. Zool., 1, 

 4, 1890, p. 527) recommends borax-carmine followed by weak alcoholic 

 solution of malachite green, with a final washing out with stronger 

 alcohol. 



390. Carmine and Picro-nigrosin (PIANESE). See Journ. Roy. 

 Mic. Soc., 1892, p. 292. 



391. Carmine and Picric acid. See 298. 



Hsematein or Hsematoxylin Combinations. 



392. Haematoxylin and Picric Acid. See 298. 



393. Haematoxylin and Eosin.- -This popular combination 

 gives results that are a3sthetically beautiful, but (for most 

 objects) is not so useful as many others, the eosin lacking 

 in electivity. Objects may be stained with hasmatoxylin 

 (either in the mass or as sections) and the sections stained 

 for a few minutes in eosin. I think it is better to take the 

 eosin weak, though it has been recommended (S'roRR, see 

 Zeit. wiss. lUik., i, 1884, p. 583) to take it saturated. Either 

 aqueous or alcoholic solutions of eosin may be used. 



HICKSON (Quart. Journ. Mic. Sci , 1893, p. 129) stains 

 sections for one hour in a strong solution of eosin in 90 per 

 cent, alcohol, washes with alcohol, and stains for twenty 

 minutes in a weak solution of haematoxylin. 



This method is most particularly recommendable for 

 embryological sections, as vitellus takes the eosin stain ener- 

 getically, and so stands out boldly from the other germinal 

 layers in which the blue of the hsematoxylin dominates. 



See also LIST (Zeit. iviss. Mik., ii, 1885, p. 148) ; BUSCH 



