TKGTJMENTARY ORGANS. 333 



(3) Huemalum for two hours, neutral orcein as above for 

 ten to twenty minutes. 



More recently UNNA advocates the process mentioned 



last . 



See alse RANVIER, Arch. Anat. Mikr., iii, 1899, p. 1. 



657. Keratohyalin.- -The keratohyalin granules of the cells 

 of the stratum granulosum are soluble in mineral acids, and 

 can be digested in pepsin. They can be stained with picro- 

 carmine, alum haematoxylin, van Gieson's mixture, or Unna's 

 Wasserblau-orcein, last . FICK (Centralb. allg. Path., xiii, 

 1902, p. 987 ; Zeit. wiss. Mik., xx, 1903, p. 222) stains sections 

 of alcohol material for three to four minutes in concentrated 

 aqueous solution of Kresylechtuiolett, differentiates in alcohol, 

 clears in xylol, and mounts in balsam. 



See also UNNA, Monatsschr. pralet. Derm., xx, 1895,. p. 69 ; the article 

 " Haut " in the Encycl. mile. Teclmik. ; and UNNA and GOLODETZ, 

 Monatsschr. pralet. Derm., xlix, 1909, p. 95 ; LAFFONT, Bibl. Anat., 1909, 

 p. 209. 



For Triciwhyalin, see GAVAZZENI, Monatsschr. prakt. Derm.,xlvii, 1908, 

 p. 229. 



658. Eleidin. To demonstrate the stratum granulosum and 

 the eleidin granules RAN VINE (Arch. Anat. Micr., iii, 1899, 

 p. 1) hardens with alcohol, stains with picro-carmine, and 

 treats with lime-water. The cells swell and show up the 

 granules, which do not change. See loc. cit., other methods 

 for the study of skin. 



Buzzi (see Encycl. mil'. Tcchnik., article " Haut ") stains 

 sections for a few minutes in a watchglassfnl of water with 

 2 to 3 drops of 1 per cent. Congo red. Similarly WEIPEN- 

 uiacn, Arch. mik. Anat., Ivii, 1901, p. 583. Other authors 

 recommend nigrosin, or Wasserblau, or orcein. 



See also JOSEPH, " Dermatohist. Technik," Berlin, 1905, 

 and DREUW, Med. Klinik, Berlin, 1907, Nos. 27 and 28. 



For Oholesterin see GOLODETZ and UNNA, Monatsschr. prakf. 

 Derm., xlvii, 1908, p. 1. 



659. Horn, Hair, and Nails Tlie elements of hairs and nails 

 may be isolated by prolonged maceration in 40 per cent, 

 potash solution, or by heating with concentrated sulphuric 

 acid. See also VON NATHUSTUS, Zool. Anz., xv, 1892, p. 395. 



