CONNECTIVE TISSUES. 353 



solution, and stains in a couple of minutes. Wash out with 

 alcohol of 70 per cent. 



With either solution the staining* must be done in a 

 covered vessel or the stain will precipitate. 



Similarly BELL, Amer. Jonrn. Anat., ix, 1909, p. 401, and 

 Anat. Bec. t iv, 1910, p. 199. 



HERKHEIMER also (Oentralb. ally. Path., xiv, 1903, p. 841 ; 

 Zeit. wiss. Mik., xxi, 1904, p. 57) recommends a saturated 

 solution of the dye in a mixture of equal parts of acetone 

 and 70 per cent, alcohol. 



He also (DeiitscJie vied. Wochenschr., xxvii, 1901, p. 607; 

 Zeit. wi#s. Mik.j xix, 1902, p. 67) has had very fine results 

 by staining for twenty minutes in a saturated solution of 

 Indophenol in 70 per cent, alcohol. 



MOLLISON (Zeit. wisa. ZooL, Ixxvii, 1904, p. 529) has had 

 good results by staining gelatin sections for a few minutes 

 in strong extract of Alkanna in 96 per cent, alcohol, and 

 mounting in glycerin or syrup. 



LORKAIN SMITH (Juurn. Path. Bact., xii, 1907, p. 1) finds 

 that Nile blue stains fatty acids blue and neutral fats reddish. 



Similarly EISENBKKG (Virchow'ts Arch., cxcix, 1910, p. 502) 

 who recommends aqueous solution of Nilblau BB. 



BENDA (ibid., clxi, 1900, p. 194) finds that free fatty acids 

 can be detected by Weigert's neuroglia mordant. See also 

 BERNER, Hid., clxxxvii, 1907, p. 360, and FISCHLER, Zeit. wiss. 

 Mik., xxii, 1905, p. 263. 



OKAJIMA (ibid., xxix, 1912, p. 67) extracts red capsicum 

 berries for some days with alcohol, and evaporates down to 

 one fifth. This stains only fatty bodies' : amongst them, 

 my el in. 



See also KINGSBURY, Anat. Rec., v, 1911, p. 313. 



710. Bone, Non-decalcified. RANVIER (Traitr, p. 297) has 

 the following : 



Bones should be plunged into water, without being allowed 

 to dry, as soon as the surrounding soft parts have been 



* For a detailed review of the whole subject, see the paper of 

 SCHAFFER in Zeit. wiss. Mik., x, 1893, p. 167, or the article " Knocheii 

 mid Z ah ne " hi Encycl. milt. Tecliui.lt. 



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