356 CHAPTER XXIX. 



to fourteen days, change frequently) and makes celloidin 

 sections. 



For decalcification of teeth see also 554 (ROUSSEAU, 

 BODECKER and FLETSCHMANN) . Bodecker finds Rousseau's 

 process not applicable to human teeth : the acid must be 

 added to the fluid celloidin. 



713. YIVANTE (Intern. Monassclir. Anat. n. Phys., ix, 1892, p. 398) 

 impregnates portions of frontal bone of four to six months calves, which 

 are not more than 3 to 4 mm. thick, by Golgi's rapid bicromate and 

 silver process. After impregnation the specimens should be decalcified 

 in von Ebner's mixture ( 562), well washed with water, and brought 

 into solution of carbonate of soda, and finally imbedded in paraffin. 

 For his cjumolem blue method see fourth edition. 



For UNDERWOOD'S gold process for teeth, and for that of LEPKOWSKI, 

 see third edition, or Anat. Anz., 1892, p. 294. 



LAW (Proc. Roy. Soc. Mcd., i, 1908, p. 45) studies nerve-endings in 

 teeth of mammals by treating paraffin sections of decalcified tissue with 

 BETHE'S molybdenum toluidin blue (details in Joitrn. Roy. Micr. Soc., 

 1908, p. 518). 



714. Bone, Decalcified (FLEMMING, Zeit. wiss. mik., 1886, 

 p. 47). Sections of decalcified bone are soaked in water, 

 dehydrated with alcohol, underpressure, dried underpressure 

 and mounted in hard balsam melted on the slide. They 

 show the lacunar system injected with air as in non-decalcified 

 sections ( 711). 



715. Stains for Cartilage and Decalcified Bone. See hereon 

 SCHAFFER in Zeit. iviss. Mik., v, 1888, p. 1 ; and Encycl. mik. 

 Technik., art. "Knochen." 



KOLLIKER (Zeit. wiss. Zool., xliv, 1886, p. 662) treats 

 sections of decalcified bone with concentrated acetic acid 

 until they become transparent, and then puts for one quarter 

 to one minute into a concentrated solution of indigo-carmine, 

 washes and mounts in glycerin or balsam. The fibres of 

 Sharpey appear red, the remaining bone substance blue. 



SCHAFFER (Zeit. wiss. Mik., v, 1888, p. 17) employed at 

 one time a safranin method modified from BOUMA (Centralb. 

 med. Wiss., 1883, p. 866), for which see previous editions. 

 He now (Encycl. mik. Tech., 1910, i, p. 762) stains sections 

 for twenty-four hours in a bath of 20 c.c. of water with one 

 drop of 1 per cent, solution of safranin (or thionin) and 



