358 CHAPTER XXIX. 



FUSARI (Arch. Ital. BioL, xxv, 1896, p. 200) makes sections of fresh 

 cartilage, puts them for twenty-four hours into 1 per cent, nitrate of 

 silver, washes, dehydrates, and exposes to the light in balsam. 



See also DISSE, Anal. Anz., xxxv, 11)09, p. 318, a stain for dentine, 

 (hsemalum followed by a mixture of Saiirerubiii and Orange G) ; and 

 RETTERER and LELIEVRE, C. 11. Soc. BioL, Ixx, 1911, p. 030. 



716. Cartilaginous Skeletons of embryos (VAN WIJHE, Proc. K. 

 Almd. Wetcnsch. Amsterdam, 1902, p. 47) may be studied by staining 

 ombryos for a week in a solution of 0'25 grm. methylen blue in lOOc.c.of 

 70 per cent, alcohol with 1 per cent, of hydrochloric acid. Wash out 

 in alcohol with 1 per cent, of hydrochloric acid until no more colour 

 comes away (about a week) and mount in balsam. The cartilage 

 remains blue, till the other tissues being colourless. 



Similarly, LUNDVALL (Anat. AHZ., xxv, 1904. p, 219, and xl, 1912, p. 

 639) using toluidin blue. 



Similarly also BAKAY (Verli. Anat.Ges., 1902, p. 248), with Bismarck 

 brown, (the embryos having been previously treated with nitric acid of 

 3 per cent.). 



