MYELIN STAINS. 409 



Sections are made, well washed in water, and brought 

 into a stain composed of 



Ha3inatoxylin . . . . 0*75 to 1 part. 



Alcohol . . . . .10 parts. 



Water . . . . 90 



Saturated solution of lithium carbonate 1 part. 

 They remain there for spinal cord, two hours ; medullary 

 layers of brain, two hours ; cortical layers, twenty-four 

 hours. 



They are then again well washed with water, and brought 

 into a decolorising solution composed of 



Borax . . . . . 2'0 parts 



Ferricyanide of potassium . . 2*5 ,, 



Water ..... 200'0 



They remain there until complete differentiation of the 

 nerves (half an hour to several hours), and are then well 

 washed with water (running, or changed several times), 

 dehydrated, and mounted in balsam. They may be 

 previously stained, if desired, with alum-carmine for the 

 demonstration of nuclei. 



The method is applicable to the study of peripheral nerves 

 as well as to nerve-centres, and also to the study of lymphatic 

 glands, skin (see SCHIEFFERDECKER, Anat. Anz. 3 ii, 1887, p. 

 680), bile capillaries, and other objects. 



The process is applicable to tissues that have been hardened in alcohol 

 or in any other way, provided that they be put into a solution of a 

 chromic salt until they become brown, before mordanting them in the 

 copper solution. 



It is not necessary that the mordanting be done in bulk. 

 MAX FLUSCH (Zeit. tms. Mik. y iii, 1880, p. 50) prefers 

 (following LICHTHEIM) to make the sections first, and mordant 

 them separately. 



VASSALE (quoted from BATON'S Hist. Untersuchungsmeth. 

 (1. Nervensy stems, Wiirzburg, 1905, p. 124) first stains the 

 sections in 1 per cent, hsematoxylin, for three to five minutes, 

 then puts for three to five minutes into saturated solution of 

 acetate of copper, and differentiates. 



782. WEIGEKT'S 1891 Method (Dent ache -mcd. Wuche 

 42, 1891, p. 1184; fait, iciss. Mil'., viii, 1891, p. 392). The 



