414 CHAPTKR XXXFIT. 



793. STRICETLCM (Arch. Mik. Anat., Ixii, 1903, p. 734) stains 

 small nerve-centre* in bulk (after mordanting in Weigerfc's 

 bichromate and fluoride mixture, 781) with Weigert's 

 haematoxylin (four to six days), washes for a couple of da}^s 

 in 70 per cent, alcohol, makes paraffin section*, and differ- 

 entiates them by the method of Weigert or Pal. 



791. BESTA (Zeit. wiss. Mile., xxiv, 1907, p. 185) mordants nerves for 

 one to three days in 100 c.c. of water with '25 of formol and 4 grins, of 

 Merck's ammonia-chloride of tin, cuts in paraffin, stains in MALLORY'S 

 hamiatoxylin and differentiates in solution of iodine in iodide of 

 potassium. 



792. Gallein. ARONSON (Centralb. med. Wits., 1890, p. 577) stains 

 sections of material hardened in liquid of Erlicki or Miiller (these must 

 be mordanted with acetate of copper) for twelve to twenty-four hours in 

 a solution of 3 to 4 c.c. of Gallein (Griibler & Co.) in 100 c.c. of water 

 with 20 of alcohol and three drops of concentrated solution of carbonate 

 of soda. They are then differentiated by the method of Weigert, or Pal. 

 Nerve-fibres red. A second stain with Methyleii blue may follow (best 

 after differentiating with permanganate). Similarly SCHROTTER 

 (Centralb. allg. Path., xiii, 1902, p. 512). 



793. SCHROTTER (Neurol. Centralb., xxi, 1902, p. 338; Zeit. wiss. 



xix, 1903, p. 381) also stains sections for two to three hours in a 5 per 

 cent, solution of sidplializarlnate of sod-t, to which is added a few drops 

 of 5 per cent, oxalic acid (enough to give an orange tint), then differ- 

 entiates until no more colour comes away, in carbonate of soda solution 

 of jo 3 oo strength, and mounts in balsam. Myelin red, on a colourless 

 ground. 



794. Toluidin Blue and Methylen Blue. HARRIS (Philadelphia 

 Med. Journ., May 14th, 1898) stains sections (of material hardened as 

 for Weigert's stain) for several hours in a 1 per cent, solution of 

 toluidin blue in 1 per cent, borax solution, and differentiates in saturated 

 aqueous solution of tannic acid. Similarly, but with methylen blue, 

 in a complicated way FRAENKEL, Neurol. Centralb., xxii, 1903, p. 76(3 

 (Zeit. wiss., Mik., xx, 1904, p. 345). 



BING ami ELLERMANN (Arch. Anat. Phys., Phys. Abth., 1901, p. 260) 

 harden in 9 parts of acetone to 1 of formol, cut without embedding, 

 stain for five to ten minutes in saturated Methylen blue, and put for one 

 or two into saturated picric acid. 



795. Other Modifications or Similar Methods. FLECHSIG, Arch. 

 Anat. Phys., Phys. Abth., 1889, p. 537 ; BREGLIA, Zeit. iviss. Mik., vii, 

 1890, p. 36; Rossi, ibid., vi, 1889, p. 182; MERCIER, ibid., vii, 181)1, 

 p. 480; HAUG, ibid., p. 153; WALSEM, ibid., xi, 1894, p. 236; ROBERTSON, 

 ibid., xiv, 1897, p. 80 (Brit. Med. Journ., 1897. p. 651). 



