440 CHAPTER XXXV. 



MALLORY (Journ. Exper. Med., 1897, p. 532) fixes tissues for four days 

 in 10 per cent, solution of formalin, then for four to eight in saturated 

 solution of picric acid (or for the same time in a mixture of the two), 

 then mordants for four to six days at 37 C. in 5 per cent, solution of 

 bichromate of ammonia, makes sections (celloidin) and stains them in 

 WEiGERT's/fcmi. stain. 



STORCH (Virchoiv's Arch., clvii, 1899, p. 127; Zeit. iviss. Mile., xvi, 

 1900, p. 475), instead of mordanting the material in bulk with the copper 

 fluid, first makes celloidin sections. 



BARTEL (Zeit. wiss. Mile., xxi, 1904, p. 18) first makes paraffin sections 

 and treats them with all the reagents without removing the paraffin, 

 until they have passed the anilin-xylol mixture, which should consist of 

 1 of anilin to 10 of xylol (or more), and be allowed to act for twelve to 

 twenty-four hours. 



SAND takes material fixed as for his neurofibril stain, p. 400, and stains 

 it as Weigert. 



See also AGUERRE and KRAUSE, Arch. Mik. Anat., clii, 1900, p. 509 ; 

 and WIMMER, Zeit. wiss. Mile., xxiv, 1907, p. 192. 



RUBASCHKIN (Arch. mik. Anat., Ixiv, 1904, p. 577) injects 

 centres of small mammals with the fixing liquid. To make 

 this, take 100 parts of 2'5 per cent, solution of bichromate of 

 potash and 0'5 to 1 of acetate of copper, boil, and add 2'5 

 to 3 of glacial acetic acid. To this (which may be kept in 

 stock) add just before use 10 per cent, of formol. Inject 

 warm, and after ten minutes dissect out and harden in the 

 liquid for five to seven days at 35 to 40 C. Dry super- 

 ficially, put for six to twelve hours in alcohol of 95 per cent., 

 and get into celloidin or paraffin. Stain sections on slide for 

 six to twelve hours in saturated aqueous solution of methyl- 

 violet B; treat for half a minute to a minute with Gram's 

 iodine in iodide of potassium (1 : 200 or 300) ; differentiate 

 in anilin or clove oil and pass through xylol into balsam. 

 Said to give very sharp results with small mammals. 



839. Benda's Methods (Neurol. Centralb., xix, 1900, p. 796, and his art 

 " Neitroglinfiirbiuig" jEncycl. Mile. Teclinik., ii, p. 308) are as follows : 

 The material is to be fixed with alcohol, and further treated with nitric 

 acid, etc., as directed for centrosomes, 651, and paraffin sections are 

 made and fixed to slides and the paraffin removed. They are then 

 mordanted and stained as directed under (6), 651 and differentiated 

 and mounted as there described. 



Glia fibres and nuclei blue, the rest red. 



Besides this, BENDA also recommends hardening and making paraffin 

 s cbions as described, then staining with the modified WE IGERT stain given 

 for central corpuscles under (n), 651 ; or, staining with HEIDENHAIN'S 



