442 CHAPTER XXXV. 



Similarly, in a very complicated way, L'HERMITTE and GTJCCIONE, 

 Semaine Medicale, xxix, 1909, No. 18, and MERZBACHEE,, Journ. f. 

 Psych, u. NeuroL, xii, 1909, p. 1 (Zeit. wlss. Mile., xxviii, 191], p. 229). ' 



See also GALESESCU, C.E. Soc. Biol., Ixv, 1908, p. 429 (sections mor- 

 danted with resorcin and stained witli methyl -violet and oxalic acid). 



842. Saurerubin. KULTSCHITZKY (Anal. Anz., viii, 1893, p. 357) 

 stains paraffin sections (of material hardened in his copper liquid, 55), 

 either for five to ten seconds with a mixture of 1 grin. Saurerubin 

 (Rubin S.), 400 c.c. 2 per cent, acetic acid, and 400 c.c. saturated solution 

 of picric acid, or for half an hour in a mixture of 3 to 5 c.c. of the above 

 stain with 100 c.c. of 96 per cent, alcohol and washes out well with 

 alcohol. Glia violet, ganglion cells and axis cylinders reddish. 



Insignificant modifications are described by POPOW, Zeit. wiss. Mile., 

 xiii, 1896, p. 358, and BURCHARDT, La Cellule, xii, 1897, p. 364. 



843. YAMAGIWA (Virclww's Arch., clx, 1900, p. 358) hardens very smctll 

 pieces of tissues for a month or more in liquid of Miiller, makes celloidin 

 sections, stains for twelve hours in saturated alcoholic solution of eosin, 

 then for four to six in saturated solution of aiiilin blue in water, and dif- 

 ferentiates in dilute alcohol with a very little caustic potash. Water, 

 alcohol, origanium oil, balsam. Neuroglia red, axis-cylinders blue. 



844. ACHUCARRO (Sol. Soc. Espan. Biol. } Madrid, 1911, p. 

 139; Zr.it. wiss. Nik., xxix, 1912, p. 238) puts sections of 

 frozen formol material into cold saturated solution of tannin, 

 warms till vapour is given off, rinses, and puts into 10 c.c. 

 of water with 6 to 8 drops of Bielschowsky's oxide of silver 

 solution (undiluted). As soon as they turn yellow they are 

 put into formol of 10 percent., and after about 10 minutes 

 washed and mounted. 



By mordanting the material with 5 per cent, acetate of 

 copper, or Weigert's mordant, and silvering by Ramon y 

 Cajal's process, he gets a stain of the amoeboid cells of the 

 cortex. 



Retina * 



845. Fixation and Hardening.- -Notwithstanding the Encycl. 

 mik. Technik., 2nd edition, p. 75, I hold that osmic acid is by 

 far the best fixing agent. The retina of small eyes is best 

 prepared by fixing the entire unopened bulb with osmium 



Besides the sources quoted in the text, see SELIGMANN, Die mikro- 

 skopischen Untersuchungsmethoden des Auyes, Berlin, S. Karger (Karl- 

 strasse 13), 1899; GREEF Anleitung zur M/hr. Untersuch. d. Auges, 

 Berlin, Hirschwald, 1898; and the Art. "Retina" in Encycl. 

 Technik., 2nd edition, p. 575. 



