446 CHAPTER XXXV. 



eight to ten hours in " Fleminiug," wash in water, decalcify 

 (twenty-four hours is enough) in 1 per cent, hydrochloric 

 acid, wash the acid out, dehydrate, and imbed in paraffin. 



PRENANT (Intern. Monatssch. Anat., ix, 1892, p. 28). Open 

 the cochlea in solution of Flemming or of Hermann, and fix 

 therein for four to five hours. Avoid decalcification as far 

 as possible, but if necessary take 1 'per cent, palladium 

 chloride. Make paraffin sections. 



Isolation preparations of the stria vascularis may be made 

 by putting a cochlea for a day into 1 per cent, solution of 

 osmic acid, then for four to five days into O'l per cent, 

 solution ; the stria may then be got away whole. 



KATZ (Zeit. wins. Mik., xxv, 1908, p. Ill) fixes the inner 

 ear, opened, for one or two hours in 30 c.c. of 0'5 per cent, 

 osmic acid with 5 drops of acetic acid, then adds 10 drops 

 of acetic acid and 60 c.c. of chromic acid (or platinum 

 chloride) of 0'5 per cent, and leaves it for four days therein. 

 He then rinses, puts for twelve to twenty-four hours into 

 pyroligneous acid or pyrogallol or tannin solution, decalcifies 

 (not necessary for mice) in 200 parts of water with 1 of 

 chromic acid and 4 to 10 of nitric or hydrochloric acid, and 

 imbeds in celloidin or sometimes paraffin. 



Similarly WITTMAACK, see 799. 



BIELSCHOWSKI and BRUUHL (Arcli. mik. Anat., Ixxi, 1907, 

 p. 27) fix the petrous in formol of 20 per cent., decalcify it 

 in nitric acid of 5 per cent., wash this out, and put back for 

 a few days into the formol, cut by the freezing method, and 

 silver by the neurofibril method 770 (24 hours in nitrate 

 of 4 per cent., but only a few minutes in the oxide bath). 



Similarly MULLENIX, Bull. Mus. Camp. Zool. Harvard Coll.., 

 liii, 1909, p. 215. 



STEIN (Anat. Anz., xvii, 1900, p. 398) decalcifies in 

 celloidin by the method of ROUSSEAU. So also KISHI (Arch. 

 in Hi. Anat., lix, 1901, p. 173). 



For staining, KANVIER (Traitc, p. 991) employs his gold 

 and formic-acid method. 



The bichromate-and-silver method of GOLGI may be em- 

 ployed with/ceaZ or new-born subjects. The methylen blue 

 intra vita-ni method has given good results. For the higher 

 vertebrates the injection method should be employed. The 

 Encycl. mik. Technik, i, p. 51 1, recommends injection of 1 c.c. 



