452 CliAl'TKU XXXVI. 



with hydrochloric acid (in preference to nitric acid). The 

 mixture 582 may also be used. If you stain with borax- 

 carmine and wash out in this mixture, the pigment will be 

 found to be removed quicker than the stain is washed out. 



LEKHOSSEK (Zeit. ids*. Zool., Iviii, 1894, p. 636; Arch. mil*. 

 A nut., xlvii, 1896, p. 45) applies the method of GOLGI to the 

 eyes of Cephalopods. 



Similarly KOPSCH (Anat. Anz. } xi, 1895, p. 362), but using 

 formol instead of the osmic acid. 



HESSE (Zeit. wins. Zool., Ixviii, 1900,, p. 418) fixes eyes of 

 Heteropoda with one of formol to 4 of water, and (p. 456) 

 bleaches those of Cephalopoda by the methods of GR KNACKER 

 and that of JANDER, 583. 



See also MERTON, ibid., Ixxix, 1905, p. 326. 



860. Eyes of Lamellibranchiata. See PATTEN, Mitth. Zool. 

 Stat. Neapel, vi, 1886, p. 733, and RAWITZ, Jena. Zeit. Naturu-., 

 xxii, 1888, p. 115, and xxiv, 1890, p. 579 (bleaches with 

 caustic soda); see 584. HESSE (op. cit., last , p. 380) 

 employs the method of JANDER for Area. He fixes the eye 

 of Pec ten in 10 per cent, formol for five minutes, followed by 

 sublimate or picro-nitric acid. 



861. Shell. Sections of non-decalcified shell are easily 

 obtained by the usual methods of grinding, or, which is often 

 a better plan, by the methods of v. KOCH or EHRKNBAUM. 

 MOSELEY (Quart. Journ. Mic. Sci. [2], xxv, 1885, p. 40) 

 decalcifies with nitric acid of 3 to 4 per cent, and then makes 

 sections. This method serves for the study of the eyes of 

 CHITONIDJE. 



862. Injection of Acephala (FLKMMING, Arch. mik.Anat., 1878, 

 p. 252) .--To kill the animals freeze them in a salt-and-ice 

 mixture, and throw them for half an hour into lukewarm 

 water. They will be found dead, and the injection-pipe may 

 be tied in the heart, and the entire animal filled and covered 

 up with plaster-of-Paris, which serves to occlude cut vessels 

 that it is not possible to tie. As soon as the plaster has 

 hardened the injection may be proceeded with. See also 

 DEWITZ, Anleit. zur Anfert. zootom. Prap. } Berlin, 1886, p. 44 

 (Anodonta) and p. 52 (Helix). 



DAKIN (Liverpool Mar. Biol. Cowm., xvii, 1909, p. 76) 



