METHODS FOR INVERTEBRATES. 459 



CERFONTAINE (Arch, de Biol., x, 1890, p. 327) injects inter- 

 stitial! v about 2 c.c. of a 1 : 500 solution of curare. 



a/ 



JAQUET (Bib. Anat., iii, 1895, p. 32) kills Lumbricus in 

 extension in 1 part of nitric acid to 125 of water. 



COLLIN (Zeit. wiss. ZooL, xlvi, 1888, p. 474) puts Criodrilns 

 Jacunm into a closed vessel with a little water, and hangs up 

 in it a strip of blotting-paper soaked in chloroform. KUKKN- 

 THAL (Die mil-. Teclmik, 1885 ; Zeit. inss. Mik,, 188(3, p. 61) 

 puts Annelids into a glass cylinder filled with water to the 

 height of 10 centimetres, and then pours 70 per cent, alcohol 

 to a depth of one to two centimeters on to the water. For 

 Opheliadae he also employs O'l per cent, of chloral hydrate 

 in sea water. 



Many marine Chastopoda may be successfully narcotised 

 (Lo BIANCO) in sea water containing 5 per cent, of alcohol, 

 or by means of the mixture 18. 



The PolyeJiieta sedentaria may sometimes be satisfactorily 

 fixed by bringing them rapidy into corrosive sublimate. 

 Cold, not hot, solutions should be taken, as heat frequently 

 shrivels up the branchiae. Eunice and Onnphi* may be treated 

 in the same way. 



Lo BIANCO advises killing Cluetopteridre, Sternaspida3, 

 Spirogr aphis, Prot/ula. by putting them for half an hour 

 into 1 per cent, chromic acid. Some of the sedentaria may 

 bo got protruded from their tubes by leaving them for some 

 hours in O'l per cent, chloral hydrate in sea water. 



For EJSIG'S methods for Capitellida? see Fauna u. Flora 

 Golf. Ncapel, xvi, 1887, p. 295. 



See also 14 (lemon juice), and the methods 20 to 20, 

 39 and 49. 



879. Blood-vessels of Annelids (KUKENTHAL, Zeit. iv-iss. Nik., 



1886, p. 61). The animals should be laid open and put for 



two or three hours into aqua rcgia (4 parts of nitric acid to 



2 of hydrochloric acid). Vessels black, on a yellow ground. 



BEEGH (Anat. Ueftc, xlv, 19i 0, p. 392, and xlix, 1900, p. 

 599) puts small Annelids for a week or more into equal parts 

 of 1 per cent, nitric acid and 1 per cent, nitrate of silver, or 

 into 50 parts of nitrate, 25 of formic acid, and 25 of water, 

 dissects out the organs and exposes to light. Marine forms 

 may be treated by HARMER'S process. 



