478 CHAPTER XXX\ 7 I. 



2 per cent, osmic acid, or its vapours (10 to 30 minutes). 

 For details see previous editions. 



LONGHI (Bull. Mus. Zool. Univ. Genova, 1892,, No. 4) kills 

 in 10 c.c. of 1 per cent, sulphate of eserin with 1 drop of 1 

 per cent, sublimate. 



SCALA (Rev. Hus. La Plata, xv, 1908, p. 57) fixes for 5 or 

 10 minutes in a mixture of 2 mg. of atropin, 10 drops of 

 formol, 10 grm. of glycerin and 50 c.c. of water. 



See also PUSCHKAREW, Zeit. wi&s. Mik., xxviii, 1911, p. 145 

 (agar process for fixing and staining Amoebae). 



FOL (Lehrb., p. 102) fixes delicate marine Infusoria (Tin- 

 iinnodea] with the perchloride of iron solution ( 80), added 

 to the water containing them, and stains with gallic acid, 

 375. 



Lo BIANCO (loc. cit., p. 444) fixes G-regarina3 with picro-sul- 

 phuric acid (one hour), Vorticella3 with hot sublimate, 

 Acinetae with sublimate in sea water, or with osmic acid, 

 Thalassicola with 0'5 per cent, chromic acid (one hour), 

 Aeanthometrae and Aulacanthae with 50 per cent, alcohol or 

 with concentrated sublimate, or by adding a little osmic acid 

 to the water. For Sphaerozoa he proceeds as BRANDT, 922. 



ZOGRAF fixes Rhizopoda and Infusoria as Rotatoria, 886, 

 but without narcotisation. 



See also FABRE-DOMERGUE, Ann. de Microgr., ii, 1889, p. 545, and 

 1890, p. 50; SCHEWIAKOFF, Biblioth. Zool., v, 1889, p. 5; Journ. Roy. 

 Mic. Soc., 1889, pp. 832, 833; ZOJA, Boll. Sci. Pavia, 1892; Zeit. whs. 

 Mik., ix, 1893, p. 485; LAUTERBORN, Zeit. wiss. Zool., lix, 1895, p. 170; 

 SCHAUDINN, ibid., p. 193 ; BALBIANI, Zool. Anz., xiii, 1890, p. 133 ; 

 KARAWAIEW, ibid., xviii, 1895, p. 286. 



921. Sections. The organisms should be strongly fixed, 

 then dehydrated and cleared, and brought into melted 

 paraffin in a small watch glass. After a feAv minutes therein 

 they are brought on a cataract needle on to a small block of 

 paraffin, and arranged there with a heated needle and 

 sectioned. They may be stained after fixation, or the 

 sections may be stained on the slide, 186 or 187. 



ENTZ (Arch. Protisterik., xv, 1909, p. 98) brings the objects 

 from clove oil into clove oil collodion of the consistency of 

 honey, then brings them in this into a funnel made of 

 paraffin, and when they have collected at the bottom of this 



