480 CHAPTER XXXVI. 



body -cavity and salivary glands of Anopheles by treating 

 them with normal salt solution containing 2 per cent, of 

 formol (pure formol produces swellings) , or in a mixture 

 of 1'5 grin, of salt and 250 c.c. of water with the white of 

 an egg. He fixes with sublimate, makes paraffin sections, 

 and stains with ha3malum or iron haBmatoxylin. He stains 

 the Sporozoites by making cover-glass preparations which 

 are allowed to dry, put for twenty-five minutes into absolute 

 alcohol, and stained b}^ the process of ROMANOWSKY, 720. 



For minute instructions for the application of this process to 

 sections, see GIUMSA, Deittsch. med. Wochenschr., xxxvi, No. 12, 

 1910; and SCHUBERG, ibid., xxxv, No. 40, 1909 (Zeit. wiss. 

 Mik, xxvii, 1910, pp. 160, 161 and 513). 



For clinical methods, see COLES, The Diseases of the 

 Shod, London, J. & A. Churchill, 1905. 



BRADFORD and PLIMMER (Quart. Journ. Micr. Sci. } xlv, 1902, 

 p. 452) fix Trypanosomes in vapour of equal parts of acetic 

 acid and 2 per cent, osmic acid, or with GTDLLAND'S formol and 

 absolute alcohol, and stain with methylen blue and eosin, 

 and mount in turpentine colophonium. 



HINDLE (Univ. Calif. Pub. Zool. } vi, 1909, p. 129) makes 

 smears on cover glasses coated with albumen, fixes for five 

 minutes in liquid of Flemming, passes through water up to 

 absolute alcohol, then for ten minutes into alcohol of 80 per 

 cent, with a good proportion of iodine in potassic iodide, then 

 into 30 per cent, alcohol, and stains with iron haBmatoxylin 

 or safranin, then with polychrome methylen blue, and lastly 

 with UNNA'S orange with tannin, and gets quickly through 

 alcohol into xylol and balsam. 



MIKCHIN (Quart. Journ. Micr. 8ci. } liii, 1909, p. 762) makes 

 cover-glass smears, fixes them with vapours of osmic acid 

 (with or without acetic acid), and mounts them dry, or in 

 balsam after fixing in liquids and various stains, amongst 

 these that of TWORT. Half-saturated solutions of neutral 

 red and Lichtgriin are mixed, the precipitate dried and dis- 

 solved to about O'l per cent, in methyl alcohol with 5 per 

 cent, of glycerin. Three parts of this are diluted with 1 of 

 water, the smears stained for an hour, differentiated with 

 UNXA'S glycerin-ether, and mounted in balsam. This stain 

 works best after fixation with sublimate. 



POLICARD (C. R. Soc. Biul. } Ixviii, 1910, p. 505) stains 



