METHODS FOE INVERTEBRATES. 483 



VAN ERMENGEM (Journ., 1894, p. 405) fixes for a few minutes 

 with a mixture of 1 part 2 per cent, osmic acid, and 2 parts 

 10 to 25 per cent, solution of tannin, washes, treats with 0'25 

 to 0'5 per cent, solution of nitrate of silver, then for a few 

 seconds with a mixture of 5 parts gallic acid, 3 of tannin, 

 10 of acetate of soda, and 350 of water, then puts back again 

 into the silver for a short time, then washes and mounts. 



See also STEPHENS, ibid., 1898, p. 685, and Gordon, ibid., 

 1899, p. 235, and the methods of TRENKMANN (Gentralb., vi, 

 1889, p. 433 ; Z&it. iciss. Mik., vii, 1890, p. 79) ; BROWN 

 (Journ. Roy. Mic. Soc., 1893, p. 268) ; JDLIEN (ibid., 1894, p. 

 403) ; SCLAVO (Zeit. wiss. Mik., xiii, 1896, p. 96) ; HESSERT 

 (ibid., p. 96); MUIR (Journ. Roy. J\lic. Soc., 1899, p. 235); 

 McCRORiE (ibid., 1897, p. 251; he stains for two minutes in a 

 mixture of equal parts of concentrated solution of night-blue, 

 10 per cent, solution of alum, and 10 per cent, solution of 

 tannic acid) ; ZETTNOW (ibid., 1899, pp. (362, 664) ; MORTON 

 (ibid., 1900, p. 131) ; WELCKE (ibid., p. 132) ; LKVADITI, C. R, 

 Soc. Biol., lix, 1905, p. 326 (for Spirochsete pallida, RAMON'S 

 neurofibril stain) ; MEIROWSKY, Miinch. tneJ. Wochenschr., 

 Ivii, 1910, No. 27; KAI.B, ibid., No. 26 (Ze!t. n-iss. Mils., 

 xxix, 1912, pp. 123, 124; both for Spirochsete). 



